Cell lysates were prepared in 2× SDS Loading Buffer (80 mM Tris pH6.8, 2% SDS, 12% glycerol, 10% β-mercaptoethanol, bromophenol blue). For the western blot shown in Figure S1B , three adult flies per sample were homogenized by grinding in 200 ul 2× SDS Loading Buffer. Lysates were boiled and clarified by centrifugation before analysis by western blotting. Primary antibodies used for western blotting were: rabbit anti-SH3PX1 (gift from Jack Dixon, 1:1500), mouse anti-α-tubulin (DSHB 12G10, 1:10,000), mouse anti-p44/42 MAPK (Erk1/2) (Cell Signaling Technology, 1:1500), rabbit anti-phosphop44/42 MAPK (Erk1/2) (Cell Signaling Technology, 1:3000), mouse anti-vinculin (Sigma-Aldrich, 1:5000), rabbit anti-EGFR (Cell Signaling Technology, 1:2000).
Mouse anti p44 42 mapk erk1 2
Manufactured by Cell Signaling Technology
Mouse anti-p44/42 MAPK (Erk1/2) is a primary antibody that recognizes the extracellular signal-regulated kinase 1 and 2 (ERK1/2) proteins. ERK1/2 are key components of the mitogen-activated protein kinase (MAPK) signaling cascade, which mediates cellular responses to various extracellular stimuli.
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Lab products found in correlation
Rabbit anti phospho p44 42 mapk erk1 2, by Cell Signaling Technology (1 mentions)
Mouse anti vinculin, by Merck Group (1 mentions)
Mouse anti α tubulin, by Cell Signaling Technology (1 mentions)
Rabbit anti egfr, by Cell Signaling Technology (1 mentions)
Rabbit anti β actin, by Abcam (1 mentions)
Rabbit anti gsk 3β, by Cell Signaling Technology (1 mentions)
Rabbit anti phospho p44 42 mapk erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions)
2 protocols using mouse anti p44 42 mapk erk1 2
1
Western Blotting of Fly Lysates
2
Antibody Immunoblotting for Synaptic Signaling
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The primary antibodies were rabbit anti-phospho-GSK-3α/β (Ser21/9; Cell Signaling, #9331), rabbit anti-GSK-3β (Cell Signaling, #9315), rabbit anti-tubulin (Tuba1; GeneTex, GTX124965), rabbit anti-phospho-p44/42 MAPK (Erk1/2; Thr202/Tyr204; Cell Signaling, #4370), mouse anti-p44/42 MAPK (Erk1/2; Cell Signaling, #9107), rabbit anti-post-synaptic density-95 (PSD-95; GeneTex, GTX80682), and rabbit anti-beta-actin (Abcam, ab8227). The secondary antibodies were HRP-conjugated anti-rabbit (Cell Signaling, 7074S) or HRP-conjugated anti-mouse (Bio-Rad). All antibodies were diluted in 3% milk/TBST, except phospho-specific antibodies, which were diluted in 2.5% bovine serum albumin/TBST.
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