The human squamous cell carcinoma cell lines, H157 and H520, adenocarcinoma cell lines H358, H441, H928, H1355, PC9, PC14, CL1-0 and CL1-5 and large-cell carcinoma cell lines H460, H661, H1299 and PC13 were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% fetal bovine serum, 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco, Grand Island, NY, USA). The human adenocarcinoma cell line, A549, was cultured in Dulbecco’s modified Eagle medium (DMEM). The medium was supplemented with 10% fetal bovine serum, 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco). All the cell lines were incubated in 5% CO2 in a 37 °C incubator. All cell lines were confirmed to be mycoplasma-negative by DNA staining. The human NSCLC cell lines A549, H157, H358, H441, H460, H520, H661, H1299, H1355 were purchased from American Type Culture Collection (Manassas, VA, USA). The H928, PC9, PC13, PC14, CL1-0, CL1-5 cell lines were a kind gift from Dr. Michael Hsiao (Genomics Research Center, Academia Sinica, Taipei, Taiwan).
H1355
Manufactured by Thermo Fisher Scientific
Sourced in United States
The H1355 is a laboratory centrifuge designed for a variety of applications. It provides consistent and reliable performance for spinning down samples in tubes and microplates. The centrifuge features a robust and durable construction to ensure long-lasting operation.
Automatically generated - may contain errors
Lab products found in correlation
H1355, by American Type Culture Collection (6 mentions)
H1975, by American Type Culture Collection (4 mentions)
H1975, by Thermo Fisher Scientific (3 mentions)
H1299, by American Type Culture Collection (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
H1993, by Thermo Fisher Scientific (2 mentions)
Mcf 7, by Thermo Fisher Scientific (2 mentions)
H1650, by Thermo Fisher Scientific (2 mentions)
Sk br 3, by Thermo Fisher Scientific (2 mentions)
H1648, by Thermo Fisher Scientific (2 mentions)
H2882, by Thermo Fisher Scientific (2 mentions)
Hcc193, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Mcf 7, by American Type Culture Collection (2 mentions)
Skbr3, by American Type Culture Collection (2 mentions)
H1650, by American Type Culture Collection (2 mentions)
H1993, by American Type Culture Collection (2 mentions)
H1648, by American Type Culture Collection (2 mentions)
Hcc193, by American Type Culture Collection (2 mentions)
6 protocols using h1355
1
Cultivation of Human Lung Cancer Cell Lines
2
Authenticated Cancer Cell Line Cultivation
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The human cancer cell lines H1975, HCC827, H2122, CHO, CHL and H1355 were purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA). PC9 cell line was purchased from the Sigma-Aldrich (St. Louis, MO, USA). A549, H3255 were purchased from Cobioer Biosciences CO., LTD (Nanjing, China). H1975, PC-9, HCC827 and EGFR mutant isogenic BaF3 cells were cultured in RPMI 1640 media (Corning, USA) with 10% fetal bovine serum (FBS) and supplemented with 2% L-glutamine and 1% penicillin/streptomycin. A549 and H1355 were cultured in F-12K Nutrient Mixture (kaighn's Modification) (Gibco, USA) with 10% FBS and supplemented with 2% L-glutamine and 1% pen/strep. H3255 was cultured in BEGM media (LONZA, USA) with 10% FBS and supplemented with 2% L-glutamine and 1% pen/strep.
We have authenticated the following cell lines through cell line short tandem repeat (STR) profiling (GENEWIZ, Suzhou, China): H1975, PC9, H3255, HCC827, A549, H2122, H1703. All cell lines matched >90% with lines listed in the ATCC, DSMZ or JCRB Cell Line Bank STR Profile Information.
We have authenticated the following cell lines through cell line short tandem repeat (STR) profiling (GENEWIZ, Suzhou, China): H1975, PC9, H3255, HCC827, A549, H2122, H1703. All cell lines matched >90% with lines listed in the ATCC, DSMZ or JCRB Cell Line Bank STR Profile Information.
3
Lung Adenocarcinoma Cell Line Maintenance and Genetic Manipulation
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Human lung adenocarcinoma cell lines, (H1355, H1573, H23, A549, H1975, and H441) were purchased from the American Type Culture Collection (ATCC) cell bank. The lung adenocarcinoma cell lines CL1-0 and CL1-5 were established and provided as a gift from Dr. Pan-Chyr Yang (National Taiwan University, Taipei, Taiwan). H1355, H1573, H23, CL1-0, H1975, H441, and CL1-5 cells were maintained in RPMI 1640 medium, supplemented with 10% fetal bovine serum (FBS) (Invitrogen, Carlsbad, CA, USA). The human lung adenocarcinoma cell line A549 was grown in F12K medium plus 10% FBS (Invitrogen) at 37 °C in a humidified atmosphere of 5% CO2. For establishing the stable PGK1 or HTATSF1 cell lines, the pGIPZ lentiviral shRNAmir system (Thermo, Waltham, MA, USA) was used with PGK1 and HTATSF1 sequences, respectively. The lentiviruses were used to infect CL1-5 cells for 2 days. Stable clones were selected with puromycin (Sigma-Aldrich, St. Louis, MO, USA) at 1 μg/ml for 2 weeks.
4
Lung Cancer Cell Line Maintenance
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The human lung adenocarcinoma cell lines H1355, PC9, H358, H928, CL1-0 CL1-1, CL1-3, and CL1-5 and squamous cell carcinoma cell lines H157 and H520 were maintained in RPMI 1640 medium (Invitrogen) supplemented with 10% fetal bovine serum (FBS). Human lung adenocarcinoma cell lines (A549, PC13, and PC14) and large cell carcinoma H1299 cells were grown in DMEM (Invitrogen) containing 10% FBS. All cells were kept under a humidified atmosphere containing 5% CO2 at 37 °C. CL1-0, CL1-1, CL1-3, and CL1-5 cell lines were established by Chu et al. at National Taiwan University Hospital and displayed progressively increased invasiveness, while PC13 and PC14 cell lines were derived from Tokyo National Cancer Centre Hospital. Other lung cancer cell lines (A549, H1355, H358, H928, H520, H157, H460, and H1299) were obtained from American Type Culture Collection.
5
Cell Line Culturing Conditions
6
Cell Line Culturing Conditions
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The cell lines MCF7, HT29, SKBR3, H441, H1355, H1993, H1648, A549, A431, H820, PC9, HCC193, H1975, H2882 and H1650 were purchased from the American Type Culture Collection (Manassas, VA) or donated by other labs. Although the cell lines were not authenticated by our lab, the application herein does not require authentication since the lines are used as standards, not at biological models. Cell lines were selected to represent a range of EGFR expression and different mutation status. H1648, A549, H1355 were routinely cultured in Dulbecco’s modified Eagle medium: Nutrient Mixture F-12 containing 10% fetal bovine serum and 1% penicillin – streptomycin (Life Technologies, Inc., Grand Island, NY). A431 and MCF7 cells were cultured in Dulbecco’s modified Eagle medium containing 10% fetal bovine serum and 1% penicillin – streptomycin (Life Technologies, Inc., Grand Island, NY). H820, H1993, H441, H2882, HCC193, PC9, H1650, H1975 cells were cultured in RPMI 1640 medium containing 10% fetal bovine serum and 1% penicillin – streptomycin (Life Technologies, Inc., Grand Island, NY). HT29 and SKBR3 were cultured in McCoys medium containing 10% fetal bovine serum and 1% penicillin – streptomycin (Life Technologies, Inc., Grand Island, NY).
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