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6 protocols using ammonium acetate

1

Analytical Method for Ilaprazole Racemate

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Ilaprazole racemate and R-lansoprazole (internal standard, IS) were kindly given by Huadong Medicine Co., Ltd., China (Fig. 1). The purity of ilaprazole racemate and R-lansoprazole was greater than 98%. The other reagents were all purchased from commerce, such as acetonitrile (AR, JA043730, German Merck KGaA), distilled water (A.S. Watson Group (Hong Kong) Ltd., China), ammonium acetate (AR, C10099621, Shanghai Macklin Biochemical Technology Co., Ltd., China).

Chemical structure of (A) R-, (B) S-ilaprazole and (C) internal standard.

Fig. 1
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2

HPLC-MS Analysis of GS441524 and QX

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HPLC-grade, LC-MS-grade acetonitrile (ACN) and methanol (MeOH) were purchased from Dikma Co., Ltd. (Massier Lane, Foothill Ranch, CA, USA). Normal saline was obtained from Qidu Pharmaceutical Co., Ltd. (Shandong, China). Hydrogen trifluoride triethylamine, tetrahydrofuran (THF), tertiary butyl dimethyl chlorosilane (TBSCl), dimethylaminopyridine (DMAP), imidazole, triethylamine, and ammonium acetate were obtained from Macklin Biochemical Technology Co., Ltd. (Shanghai, China). Formic acid, phosphoric acid, N,N′-carbonyldiimidazole (CDI), dicyclohexylcarbodiimide (DCC), dimethyl formamide (DMF), and isobutyric acid were purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). GS441524 (GS441, MW291.3) and 6,7-dimethyl-2,3-bis(2-pyridyl) quinoxaline (QX, MW312.4) were obtained from APExBIO Co., Ltd. (Houston, TX, USA) and JiSiEnBei International Business Co., Ltd. (Hong Kong, China), respectively.
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3

Synthesis and Characterization of HA-DAS Polymer

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HA was purchased from Huaxi Biotechnology Co. Ltd. (Jinan, China, MW: 8814 Da), DAS and OLA were provided by Macklin Co. Ltd. (Shanghai, China), EDCI and DMAP were purchased from Aladdin Co. Ltd. (Shanghai, China). Dimethyl sulfoxide (DMSO), glacial acetic acid and ammonium acetate were obtained from Macklin Co. Ltd. (Shanghai, China), Lyso-tracker Red was purchased from Beyotime Biotechnology Co. Ltd. (Shanghai, China). HA-DAS polymer was prepared by our research group. Reagents such as methanol and acetonitrile were chromatographic grade and supplied by TEDIA (Ohio, USA).
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4

Synthesis and Characterization of Fluorescent Benzaldehyde Derivatives

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All reagents and chemicals were used directly without any further purification. Polystyrene and acetyl chloride were purchased from China Pharmatech Ltd. (Shanghai, China). 4-(Diethylamino)-2-hydroxybenzaldehyde, malonitrile and ammonium acetate were obtained from Macklin (Shanghai, China). The ionic solutions were prepared by dissolving their relative sodium and chloride salts, respectively. Deionized water was employed in all experiments in this study.
The 1H- and 13C-NMR spectra were measured using an AV-600 spectrometer (Bruker, Switzerland) with tetramethylsilane (TMS; δ = 0 ppm) as an internal reference. The UV-visible absorption experiments were performed on a UV-1800 spectrophotometer (Shimadzu, Japan). Fluorescence spectra were obtained from an F-7000 spectrophotometer (Hitachi, Japan). The time-resolved fluorescence spectra were recorded on the transient fluorescence spectrometer (Edinburgh-FLS1000, England). The fluorescence quantum yields were measured by HORIBA FluoroMax-4P (HORIBA Jobin Yvon).
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5

Simultaneous Determination of Eight Drugs

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Metformin Hydrochloride (C4H11N5·HCl, purity 100.0%), glyburide (C23H28ClN3O5S, purity > 99.9%), tolbutamide (C12H18N2O3S, purity 98%), enalapril maleate (C20H28N2O5·C4H4O4, purity 99.8%), indapamide (C16H16ClN3O3S, purity 98.2%), nifedipine (C17H18N2O6, purity 99.8%), atorvastatin calcium (C66H68CaF2N4O10·3H2O, purity 94.9%), and reserpine (C33H40N2O9, purity > 99.9%) were purchased from national institutes for food and drug control (Beijing, China). Glyburide-d11 (C23H17ClD11N3O5S, purity 98%) was purchased from Toronto Research Chemicals (Toronto, ON, Canada). Methanol (MeOH), acetonitrile (ACN), and methyl tert butyl ether (MTBE) were of HPLC grade and purchased from Fisher Scientific (Pittsburgh, PA, USA). Acetic acid was obtained from Guangzhou chemical reagent factory (Guangzhou, China). Ammonium acetate was purchased from Macklin (Shanghai, China). Ultrapure water was freshly prepared using the Milli-Q Advantage A10 system (Milli-Q Reference, Millipore, Boston, MA, USA). Blank rat plasma samples were harvested from healthy adult rats supplied by the Laboratory Animal Center of Sun Yat-sen University (Guangdong, China).
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6

Antioxidant Capacity Evaluation of AMR

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AMR was purchased from Huadong Chinese Medicine Co., Ltd. (Zhejiang, China) and identified by Dr. Shuili Zhang of Zhejiang Chinese Medical University. Anthrone and ammonium acetate were purchased from Macklin Biochemical Technology Co., Ltd. (Shanghai, China). Glucose, arabinose and galactose were purchased from Chengdu DeSiTe Biological Technology Co., Ltd. (Sichuan, China). 2,2-Diphenyl-1-1-picrylhydrazyl radical and 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) were purchased from Chengdu Push Biotechnology Co., Ltd. Microporous membranes were purchased from SORFA Life Science Co., Ltd. (Beijing, China). The Ferric Reducing Antioxidant Power kit was purchased from Beyotime Institute of Biotechnology (Jiangsu, China). Chromatography grade acetonitrile was obtained from TEDIA (Fairfield, OH, USA). Deionized water was produced with a Millipore water purification system (Millipore Co., Ltd., Billerica, MA, USA).
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