Biocatalyst

Manufactured by Bruker

Sourced in United States

Biocatalyst is a laboratory equipment product offered by Bruker. It is designed to facilitate biocatalytic processes, which involve the use of enzymes or other biological catalysts to carry out chemical reactions. The core function of Biocatalyst is to provide a controlled environment and the necessary tools for researchers and scientists to study and optimize biocatalytic processes.

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Lab products found in correlation

Scanasyst fluid cantilever, by Bruker (1 mentions) Eclipse ti inverted microscope, by Nikon (1 mentions)

3 protocols using biocatalyst

Aconitase and GroEL/ES Visualization

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The induced samples of E. coli cells expressing aconitase and GroEL/ES grown at different temperatures (i.e., 37 °C and 25 °C) were centrifuged and pellets were resuspended in 0.5 M Tris (pH 7.5) buffer. 5 µL of the sample was laid on freshly peeled mica sheet (1 cm × 1 cm) and allowed to air dry. The samples were subjected to tapping mode analysis in atomic force microscope (Biocatalyst, Bruker Inc., USA).

Ravitchandirane G., Bandhu S, & Chaudhuri T.K. (2022). Multimodal approaches for the improvement of the cellular folding of a recombinant iron regulatory protein in E. coli. Microbial Cell Factories, 21, 20.

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Atomic Force Microscopy of PEM Films

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PEM films in Hepes/NaCl were imaged using a BioCatalyst (Bruker AXS SAS, Palaiseau, France) equipped with a 150 μm scanner in the intermittent-contact mode (512 × 512 pixels² at line rates of 1 Hz). We used a ScanAsyst-Fluid cantilever (Bruker, resonance frequency of 150 kHz, spring constant of 0.7 N/m). The root mean squared roughness R_RMS was calculated from 5 × 5 μm² AFM images. The PEM films without SDF-1α were dipped into the 1 mM HCl solution prior to imaging and then thoroughly rinsed in the Hepes-NaCl buffer.

Dalonneau F., Liu X.Q., Sadir R., Almodovar J., Mertani H.C., Bruckert F., Albiges-Rizo C., Weidenhaupt M., Lortat-Jacob H, & Picart C. (2014). The effect of delivering the chemokine SDF-1α in a matrix-bound manner on myogenesis. Biomaterials, 35(15), 4525-4535.

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Visualizing E. coli Aconitase and GroEL/ES

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The induced samples of E. coli cells expressing aconitase and GroEL/ES grown at different temperatures (i.e. 37°C and 25°C) were centrifuged and pellets were resuspended in 0.5M Tris (pH 7.5) buffer. 5µL of the sample was laid on freshly peeled mica sheet (1 cm X 1cm) and allowed to air dry. The samples were subjected to tapping mode analysis in atomic force microscope (Biocatalyst, Bruker Inc., USA). To study the cells under phase contrast microscope, 4µL of the sample was applied on a clean glass slide with a cover slip and viewed with 1000x magni cation lens under oil-immersion using Nikon Eclipse Ti inverted microscope (Nikon, Japan).

Ravitchandirane G., Bandhu S., & Chaudhuri T.K. (2021). Multimodal Approaches for the Improvement of the Cellular Folding of a Recombinant Iron Regulatory Protein in E. Coli.

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