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8 protocols using magnesium sulfate

1

Organic Synthesis Reagents Database

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Dichloromethane (CH2Cl2), chloroform (CHCl3, 99.94%), tetrahydrofuran (THF) (C4H8O, 99.94%) and ammonium hydroxide (NH4OH, 24.06%) were received from Lach-Ner s.r.o., Neratovice, Czech Republic. α-bromoisobutyryl bromide (C4H6Br2O, >98.0%), 3-thiopheneethanol (C6H8OS, 98.0%) and N,N,N′,N″,N″-pentamethyldiethylenetriamine (PMDETA) (C9H23N3, >99.0%) were recieved from Tokyo Chemical Industry, Tokyo, Japan. Nitromethane (CH3NO2, 98+%), copper(I) bromide (CuBr, 98%), dibutyltin dilaurate (DBTDL, [CH3(CH2)3]2Sn[O2C(CH2)10CH3], iron(III) chloride, anhydrous (FeCl3, 98%) and ethyl isocyanate (C3H5NO, 98%) were received from Alfa Aesar, Haverhill, MA, USA. Triethylamine (C6H15N, 99%), 3,4-ethylenedioxythiophene (EDOT, 99.0%) silica gel (SiO2) and 2-hydroxyethyl acrylate (C5H8O3, 97%) were received from Acros Organics, Geel, Belgium. Magnesium sulfate (MgSO4, 99.4%) was received from VWR Chemicals, Leuven, Belgium. Acetone (C3H6O, 98.0%) and ethanol (C2H5OH, 96.0%) were received from Gram-mol, Zagreb, Croatia. Methanol (CH3OH, 99.9%) was received from Carlo Erba Reagents, Val-de-Reuil, France and diethyl eter (C4H10O) was received from Macron Fine Chemicals, Radnor, PA, USA. All the solvents were used as received.
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2

Graphene Oxide Wastewater Treatment

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Graphene oxide (Trade name: Graphenea Graphene oxide) was purchased from Graphenea, Inc. Sodium sulfate (Na2SO4), Sodium chloride (NaCl) Magnesium sulfate (MgSO4), Magnesium Chloride (MgCl2), Sulfuric acid (H2SO4), Sodium hydroxide (NaOH) and Potassium chloride (KCl) were purchased from VWR. The PVDF substrate used in the study was a PV200 membrane with an effective pore size of 110 nm, obtained from Nanostone-Water Co. Produced water samples were provided by Chevron Corp. Sample was collected in the process after solids and oil separation including gravity separation, and induced gas floatation. The samples were allowed to settle in the laboratory for an extended period. It is expected that most the dispersed oil was removed; however, testing for submicron oil droplets was not conducted. It is possible that some of the dissolved organics may be finely dispersed droplets. Reagent grade perfluorooctanoic acid (PFOA, 97% purity) was purchased from Alfa Aesar (lot# 10212100). Water used at all stages of the experiments was purified (final resistivity <18.2 MΩ, TOC<1 ppb) using a Purelab flex water purifier obtained from ELGA Lab water. All reagents used in the study were of reagent grade and were used as received.
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3

Synthesis and Characterization of Bioactive Gelators

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N-Boc-l-phenylalanine, l-phenylalanine methyl ester hydrochloride, d-phenylalanine
methyl ester hydrochloride, and 2-(naphthalen-2-yloxy)acetic acid
were obtained from Sigma-Aldrich; glucono-δ-lactone, 1,4-dioxane,
and lithium hydroxide were purchased from Alfa Aesar; diethyl ether, N-methylmorpholine, sodium hydroxide, and hydrochloric acid
were received from Honeywell; acetonitrile, chloroform, tetrahydrofuran,
and sodium chloride were acquired from Fisher Scientific; hydrogen
chloride (ca 4 mol/L in 1,4-dioxane) was obtained from Tokyo Chemical
Industry; magnesium sulfate, dichloromethane, trifluoroacetic acid,
and isobutyl chloroformate were purchased from VWR International,
Fluorochem, and Thermo Scientific, respectively. All chemicals were
used directly without further treatment. Deionized water was used
throughout this research.
Full synthetic protocols and characterization
data for the two gelators used here are described in the Supporting
Information (Figures S1–S18).
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4

Thermoset Polymer Synthesis and Characterization

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Vanillin
(V) (99%), ethylene carbonate (EC)
(98%), potassium carbonate (K2CO3) (≥99%), N,N-dimethylformamide (DMF) (≥99%),
methacrylic anhydride (MAA) (94%), ethylenediamine (ED) (≥99%),
2,2′-(ethylenedioxy)bis(ethylamine) (Dom) (>97%), phenylbis(2,4,6-trimethylbenzoyl)phosphine
oxide (BAPO) (97%), and deuterium dimethyl sulfoxide (DMSO-d6) (99.9% atom % D) were purchased from Sigma-Aldrich
and used without any additional purification. 4-(Dimethylamino)pyridine
(DMAP) (≥99.0%, Fluka), magnesium sulfate (99%, VWR), sodium
bicarbonate (≥99%, Merck), sodium hydroxide (NaOH) (≥99%,
Fisher Scientific), ethyl acetate (EtOAc) (≥99%, VWR), dichloromethane
(DCM) (≥99%, Fisher Scientific), hydrochloric acid (HCl) (37%),
and acetone (≥99.5%, VWR) were all used as received. Sodium
lignosulfonate for the coating of the thermosets and 2,2-diphenyl-1-picrylhydrazyl
(DPPH°) for the antioxidant assay were acquired from Tokyo Chemical
Industry Co., Ltd.
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5

Preparation of Cell Culture Media

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Calcium chloride, calcium nitrate, glucose, HEPES, magnesium chloride, magnesium sulfate, potassium chloride, sodium chloride, and sodium bicarbonate were purchased from VWR (Mississauga, Canada). All other reagents were purchased from either Fisher Scientific (Ottawa, Canada) or Sigma-Aldrich (Oakville, Canada).
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6

Chemical Standards Preparation for Analytical Assays

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Citric acid, nicotinic acid and acrylamide standard (≥ 99%) were obtained from Merck (Darmstadt, Germany), calcium lactate and sodium chloride from Scharlab (Barcelona, Spain). Glycine, L (+) potassium sodium tartrate tetrahydrate, formic acid (99-100% purity) and magnesium sulfate were purchased from VWR (Fontenay-sous-Bois, France). 3.5-dinitrosalicylic acid (DNS) and hexane were from Panreac (Barcelona, Spain). Primary secondary amine (PSA) was obtained from Supelco (Bellefonte). Double distilled water was prepared for chromatographic use (Milli-Q, Millipore Corp., Bedford, MA) . All chemicals used were analytical grade, and those used for chromatographic analysis were HPLC grade. The acrylamide standard was purchased from Merck (Germany), the stock solution (1mg mL -1 ) was prepared by dissolving 100 mg of the acrylamide in 100 mL of acetonitrile, and was kept at 4ºC until use. All working solutions were prepared daily by serial dilution in acetonitrile.
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7

Synthesis and Characterization of Polymer Materials

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Naphthalene, p-methoxyphenol, triethylbenzylammonium chloride (TEBACl), methacryloyl chloride, paraformaldehyde, ethylene chloride, chlorosulfonic acid, methylene chloride, methyl methacrylate, triethylamine (TEA), and 2-hydroxyethyl methacrylate were purchased from Sigma-Aldrich (Germany). Sulfuric acid, sodium bicarbonate, sodium chloride, tin(II) chloride hydrate, hexane, chloroform, hydrochloric acid, potassium hydroxide, sodium hydroxide, acetic acid, styrene, thiourea, ethanol, benzene, ammonium chloride, magnesium sulfate, cyclohexane, dichloromethane (DCM), methanol (MeOH), acetone, acetonitrile (ACN), and tetrahydrofuran (THF) were obtained from Avantor Performance Materials (Gliwice, Poland). α,α’-Azoiso-bis-butyronitrile (AIBN) was purchased from Merck (Darmstadt, Germany). The 4,4′-thiobisbenzenethiol (TBT) was synthesized at the Department of Polymer Chemistry (Lublin, Poland). All chemicals were used as received.
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8

Oxylipin Analysis in Cell Culture

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High performance liquid chromatography- (HPLC-) grade acetonitrile, HPLC-grade methanol, formic acid, sodium selenite, insulin, heparin, transferrin, ethylenediaminetetraacetic acid (EDTA), triphenylphosphine (TPP), sodium selenite, soybean lipoxidase type V, and linoleic acid were purchased from Sigma–Aldrich (St. Louis, MO). Diethyl ether and butylated hydroxy toluene (BHT) were purchased from ACROS Organics (Fair Lawn, NJ). YOPRO-1 and propidium iodide stains were from Thermo Fisher Scientific (Waltham, MA). Antibiotics/antimycotics, trypsin-EDTA, glutamine, and bovine collagen were from Life Technologies (Carlsbad, CA). All predesigned bovine TaqMan® primers were purchased from Applied Biosystems (Foster City, CA). Deuterated oxylipid standards, nondeuterated oxylipid standards, and indomethacin were purchased from Cayman Chemical (Ann Arbor, MI). Magnesium sulfate was purchased from Avantor Performance Materials, Inc. (Central Valley, PA), and sodium borate from Fisher Science Education (Nazareth, PA). Fetal bovine serum was purchased from Hyclone Laboratories, Inc. (Logan, Utah). The HEPES buffer, HAM's F-12k, and RPMI 1640 were from Corning Inc. (Corning, NY).
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