Reactions were performed in triplicate on cDNA samples in 96-well optical plates on an ABI Prism StepOne System (ThermoFisher, U.K.). It consisted of 2 μL of cDNA, 7 μL of PCR-grade water, 10 μL of 2 × TaqMan Universal PCR Master Mix (Applied Biosystems, Foster City, CA, USA), 1 μL of Taqman specific assay probes (Applied Biosystems). Data were recorded using SDS software (v 2.3; PE Applied Biosystems). Advanced miRNA Taqman probes (ThermoFisher) were used for all miR qPCR assays.
Taqman specific assay probes
Manufactured by Thermo Fisher Scientific
Sourced in United States
TaqMan specific assay probes are oligonucleotide sequences designed to hybridize to a specific target sequence within a DNA molecule. These probes are labeled with a fluorescent reporter dye and a quencher dye, which allows for the detection and quantification of the target sequence during real-time PCR amplification.
Automatically generated - may contain errors
2 protocols using taqman specific assay probes
1
Quantitative miRNA Expression Analysis
2
qPCR Assay for Gene Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Reactions were performed in triplicate on cDNA samples in 96-well optical plates on an ABI Prism StepOne System (ThermoFisher). Reactions consisted of 2 μL cDNA, 7 μL PCR-grade water, 10 μL 2× TaqMan Universal PCR Master Mix (Applied Biosystems), 1 μL TaqMan-specific assay probes (Applied Biosystems) for PSA, and RPL19 or SYBR green primers for AR, AR-UTR (variants), β-actin (ACTB) and GAPDH (for primer details, see Supplementary Table 2, see section on supplementary materials given at the end of this article). Parameters were 50°C for 2 min, 95°C for 10 min, 40 cycles of 95°C for 15 s and 60°C for 1 min. Data were recorded using Sequence Detector Software (SDS version 2.3; PE Applied Biosystems). Levels were normalised to GAPDH, β-actin and RPL19.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!