The primary antibodies used for Western blotting were anti-mNeon (Chromotek, #32f6) and anti-α-tubulin (Sigma, T9026). The secondary antibody used for Western blotting was HRP–conjugated anti-mouse (Fisher, #PRW4021). The primary antibodies used for immunofluorescence staining were as follows: anti-Phospho-myosin II Light Chain 2 (P-MLC; Cell Signaling Technology, #3671), anti-ZO-1 (Invitrogen, #61-7300), and anti-E-cadherin (Developmental Studies Hybridoma Bank, #5D3-C). The secondary antibodies used for immunofluorescence staining were as follows: anti-rabbit-Alexa Fluor 647 (Invitrogen, #A-21244), anti-rabbit-Alexa Fluor 568 (Invitrogen, #A-11011), and anti-mouse-Alexa Fluor 568 (Invitrogen, #A-11004).
Anti mneon
Manufactured by Proteintech
The Anti-mNeon is a primary antibody that specifically binds to the mNeon Green fluorescent protein. mNeon Green is a bright, monomeric, and photostable fluorescent protein derived from Branchiostoma lanceolatum. The Anti-mNeon antibody can be used to detect and visualize the expression of mNeon Green in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti mouse alexa fluor 568, by Thermo Fisher Scientific (1 mentions)
Anti e cadherin, by Developmental Studies Hybridoma Bank (1 mentions)
Anti rabbit alexa fluor 647, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 568 anti rabbit, by Thermo Fisher Scientific (1 mentions)
Anti α tubulin, by Merck Group (1 mentions)
Anti zo 1, by Thermo Fisher Scientific (1 mentions)
Iblot 2 dry blotting system, by Thermo Fisher Scientific (1 mentions)
Anti strep, by Merck Group (1 mentions)
Bcip nbt alkaline phosphatase substrate, by Merck Group (1 mentions)
Anti flag, by Merck Group (1 mentions)
2 protocols using anti mneon
1
Antibody Labeling for Cellular Imaging
2
Western Blot Protein Detection
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After SDS-PAGE, gels were transferred onto polyvinylidene difluoride (PVDF) membranes using an iBlot 2 Dry Blotting System (Invitrogen). The membranes were blocked for one hour in TBS buffer (200 mM NaCl, 20 mM Tris pH 7.5) supplemented with 0.1% Tween-20 and 4% milk powder (anti-mScarlet and anti-FLAG) or 4% bovine serum albumin (anti-mNEON and anti-Strep). The primary antibodies (anti-mNEON from Chromotek diluted 1:500, anti-mScarlet from Chromotek diluted 1:2000, anti-Strep from IBA diluted 1:1000, and anti-FLAG from Sigma-Aldrich diluted 1:1000) were incubated for two hours at room temperature under gentle agitation. The PVDF membranes were washed three times in TBS-0.01% Tween-20 for 10 minutes. The secondary antibody, IgG conjugated with Alkaline Phosphatase (Sigma) was diluted 1:5000 and incubated for 1 hour at room temperature under gentle agitation. The PVDF membranes were washed again twice in TBS-0.01% Tween-20, and once in TBS buffer. The membranes were developed using BCIP/NBT alkaline phosphatase substrate (Sigma).
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