Log In Sign up
The largest database of trusted experimental protocols

Vivaspin devices

Manufactured by GE Healthcare
Visit Supplier

Vivaspin devices are centrifugal concentrators used for the rapid concentration and desalting of biological samples. They feature a microporous membrane that retains molecules above a certain molecular weight while allowing smaller molecules to pass through. Vivaspin devices are designed to efficiently concentrate and purify a variety of samples, including proteins, peptides, and nucleic acids.

Automatically generated - may contain errors

Lab products found in correlation

Micrococcal nuclease, by Merck Group (2 mentions) Model 491 preparative cell, by Bio-Rad (2 mentions)

Spelling variants of this product

Vivaspin (51 citations)

4 protocols using vivaspin devices

1

Antibody-Drug Conjugate Synthesis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The purified Ig(B12) was conjugated with MMAE using a protease-cleavable valine–citruline linker with a carbonyl acrylic acid head-group. In brief, interchain disulfide bonds of a full-length IgG(B12) were reduced using excess amounts of DTT and reacted with 60 equivalents of carbonyl acrylic acid–valine–citruline–MMAE molecule [25 (link)]. The reaction was stirred at 37 °C for 8–10 h. The antibody–drug conjugate was then purified using a desalting column and concentrated using Vivaspin devices (GE Healthcare). The average drug-to-antibody ratio was 4:1 as determined by native mass spectrometry.
Akkapeddi P., Fragoso R., Hixon J.A., Ramalho A.S., Oliveira M.L., Carvalho T., Gloger A., Matasci M., Corzana F., Durum S.K., Neri D., Bernardes G.J, & Barata J.T. (2019). A fully human anti-IL-7Rα antibody promotes antitumor activity against T-cell acute lymphoblastic leukemia. Leukemia, 33(9), 2155-2168.
+ Open protocol
+ Expand
2

Isolation and Purification of Native Mononucleosomes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Native human mononucleosomes were prepared according to established protocols28 with minor modifications. HeLa cells were lysed in 1.5 mM MgCl2, 10 mM KCl, 0.5 mM DTT, 0.5 mM phenylmethanesulfonylfluoride (PMSF), 10 mM Hepes-NaOH, pH 7.9using a 40-ml Dounce homogenizer (Wheaton). Nuclei, harvested by gentle centrifugation, weredigested with 0.5 U/ml micrococcal nuclease (Sigma-Aldrich) in 4 volumes 0.34 M sucrose, 3 mM CaCl2, 60 mM KCl, 0.5 mM PMSF and 50 mM Tris-HCl, pH 7.5for 10 min at 37 °C. The reaction was stopped with 50 mM EDTA, and the nucleosomes, extracted by addition of 0.5 M NaCl, were dialyzed overnight at 4 °C against 650 mM NaCl, 2 mM EDTA, 1 mM β-mercaptoethanol, and 20 mM Hepes, pH 7.4. Nucleosomes were isolated by size exclusion chromatography through a Superdex-200 column operated in 650 mM NaCl, 1 mM EDTA and 20 mM Tris-HCl, pH 7.5.For the use in strand transfer assays (Fig. 1a), nativenucleosomes, purified by size exclusion chromatography and dialyzed overnight against 100 mM NaCl, 1 mM EDTA and 20 mM Tris-HCl, pH 7.5, were separated by native 8% PAGE in a Model-491 preparative cell (Bio-Rad Laboratories). Nucleosomes were concentrated to 3 mg/ml using Vivaspin devices (GE Healthcare) and stored on ice.
Maskell D.P., Renault L., Serrao E., Lesbats P., Matadeen R., Hare S., Lindemann D., Engelman A.N., Costa A, & Cherepanov P. (2015). Structural basis for retroviral integration into nucleosomes. Nature, 523(7560), 366-369.
+ Open protocol
+ Expand
3

Antibody-Drug Conjugation Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The purified Ig(B12) was conjugated with MMAE using a protease-cleavable valine-citruline linker with a carbonyl acrylic acid head-group. In brief, inter-chain disulfide bonds of a full-length IgG(B12) were reduced using excess amounts of DTT and reacted with 60 equivalents of carbonylacrylic acid-valine citruline-MMAE molecule(25 (link)). The reaction was stirred at 37° C for 8-10hrs. The antibody-drug conjugate was then purified using a desalting column and concentrated using Vivaspin devices (GE Healthcare). The average drug-to-antibody ratio was 4:1 as determined by native mass-spectrometry.
Akkapeddi P., Fragoso R., Hixon J.A., Ramalho A.S., Oliveira M.L., Carvalho T., Gloger A., Matasci M., Corzana F., Durum S.K., Neri D., Bernardes G.J, & Barata J.T. (2019). A fully human anti-IL-7Rα antibody promotes antitumor activity against T-cell acute lymphoblastic leukemia. Leukemia, 33(9), 2155-2168.
+ Open protocol
+ Expand
4

Isolation and Purification of Native Mononucleosomes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Native human mononucleosomes were prepared according to established protocols28 with minor modifications. HeLa cells were lysed in 1.5 mM MgCl2, 10 mM KCl, 0.5 mM DTT, 0.5 mM phenylmethanesulfonylfluoride (PMSF), 10 mM Hepes-NaOH, pH 7.9using a 40-ml Dounce homogenizer (Wheaton). Nuclei, harvested by gentle centrifugation, weredigested with 0.5 U/ml micrococcal nuclease (Sigma-Aldrich) in 4 volumes 0.34 M sucrose, 3 mM CaCl2, 60 mM KCl, 0.5 mM PMSF and 50 mM Tris-HCl, pH 7.5for 10 min at 37 °C. The reaction was stopped with 50 mM EDTA, and the nucleosomes, extracted by addition of 0.5 M NaCl, were dialyzed overnight at 4 °C against 650 mM NaCl, 2 mM EDTA, 1 mM β-mercaptoethanol, and 20 mM Hepes, pH 7.4. Nucleosomes were isolated by size exclusion chromatography through a Superdex-200 column operated in 650 mM NaCl, 1 mM EDTA and 20 mM Tris-HCl, pH 7.5.For the use in strand transfer assays (Fig. 1a), nativenucleosomes, purified by size exclusion chromatography and dialyzed overnight against 100 mM NaCl, 1 mM EDTA and 20 mM Tris-HCl, pH 7.5, were separated by native 8% PAGE in a Model-491 preparative cell (Bio-Rad Laboratories). Nucleosomes were concentrated to 3 mg/ml using Vivaspin devices (GE Healthcare) and stored on ice.
Maskell D.P., Renault L., Serrao E., Lesbats P., Matadeen R., Hare S., Lindemann D., Engelman A.N., Costa A, & Cherepanov P. (2015). Structural basis for retroviral integration into nucleosomes. Nature, 523(7560), 366-369.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!