Paraffin-embedded tissue sections were processed for immunofluorescence staining using the avidin/biotin-based peroxidase system (Vectastain ABC HRP Elite Kit; Vector Laboratories, Burlingame, CA, USA). Next, the sections were incubated with anti-F4/80 primary antibody (1:200; ab111101; Abcam) overnight at 4 °C, followed by incubation with a horseradish-peroxidase (HRP)-conjugated anti-mouse IgG secondary antibody (A10551; Invitrogen, Carlsbad, CA, USA) for 1 h at room temperature.
Anti f4 80 primary antibody
Manufactured by Abcam
Sourced in United States, United Kingdom
The Anti-F4/80 primary antibody is a laboratory reagent used for the detection and identification of the F4/80 protein, which is expressed on the surface of mature mouse macrophages. This antibody can be used in various immunological techniques, such as flow cytometry, immunohistochemistry, and Western blotting, to aid in the study of macrophage biology and function.
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Lab products found in correlation
2 protocols using anti f4 80 primary antibody
1
Immunofluorescence Staining of Paraffin-Embedded Tissues
2
Immunofluorescence Staining of F4/80+ Macrophages in KOA Mouse Joints
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Frozen sections of KOA model mouse joint with a layer thickness of 10 µm were permeabilized in 1% Triton X-100 (Sigma-Aldrich, USA) for 5 minutes, then blocked nonspecific antigen site with goat serum (Sigma-Aldrich, USA) at room temperature for 30 minutes. Incubated with Anti-F4/80 primary antibody (ab6640, Abcam, UK; 1:200) for 12 h at 4°C. Then, sections were washed and incubated with Alexa Flour 488 Goat Anti-Mouse IgG (Yeasen, Shanghai, China; 1:200) for 1.5 h at room temperature in the dark, washed and sealed with anti-fluorescence quenching mounting solution (containing 4,6-diamidino-2-phenylindole, DAPI) (P0131, Beyotime Biotechnology, Shanghai, China). Cell images were captured using the Nikon laser scanning confocal microscope (A1 Plus, Nikon, Japan). The co-localization characterized by Pearson’s coefficient was evaluated using ImageJ (ImageJ software, USA).
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