RNA pulldown was performed as previous described [7 (link), 26 (link), 49 (link)]. Biotinylated SALIS transcript and EGFP RNAs were transcribed using a MAXIscript T7/T3 in vitro transcription kit (Ambion) and Biotin RNA labeling Mix (Roche).
Maxiscript t7 t3 in vitro transcription kit
Manufactured by Thermo Fisher Scientific
The MAXIscript T7/T3 in vitro transcription kit is a tool used to synthesize RNA from DNA templates. It provides the necessary components, including the T7 or T3 RNA polymerase, to generate high yields of RNA transcripts in an in vitro setting.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using maxiscript t7 t3 in vitro transcription kit
1
RNA Pulldown for Biotin-Labeled Transcripts
2
RNA Pulldown Protocol for PURPL Transcript
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RNA pulldown was performed as previous described [38 (link), 39 (link)]. Biotinylated PURPL transcript and EGFP RNAs were transcribed using a MAXIscript T7/T3 in vitro transcription kit (Ambion) and Biotin RNA labelling Mix (Roche).
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