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Accucount 5.27 μm blank particles

Manufactured by Spherotech
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The AccuCount 5.27 μm Blank Particles are a reliable and precise laboratory tool used for various applications. These particles are designed to provide a consistent and accurate size reference, enabling precise measurements and calibrations. The core function of these particles is to serve as a standard for size verification and quality control procedures in various scientific and industrial settings.

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Lab products found in correlation

Q vd oph, by Merck Group (1 mentions) Anti cd19 magnetic beads, by Miltenyi Biotec (1 mentions) Interleukin 7 (il 7), by Thermo Fisher Scientific (1 mentions) Anti igm fab2 fragments, by Jackson ImmunoResearch (1 mentions) Interleukin 4 (il 4), by Thermo Fisher Scientific (1 mentions) Anti cd43 magnetic beads, by Miltenyi Biotec (1 mentions) Anti cd40 hm40 3, by BioLegend (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions)

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AccuCount blank particles AccuCount Particles

2 protocols using accucount 5.27 μm blank particles

1

Proliferation of Rag1-/- pro-B cells

Cited 1 time
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Rag1−/− pro-B cells were purified using anti-CD19 magnetic beads (Miltenyi). Cells were cultured at 1×106 cells/ml in 96-well plates in the presence of 10 ng/ml IL-7 (Peprotech). After 24, 48, and 72 hours, proliferation was measured by flow cytometry using AccuCount 5.27 μm Blank Particles (Spherotech). Q-VD-OPH (Sigma Aldrich) was used at 100 μM. For transduction, Rag1−/− pro-B cells were cultured for 24 hours prior to transduction with MLP or MLP-shp53 retroviral vectors, as previously described (19 (link), 25 (link)).
Fahl S.P., Harris B., Coffey F, & Wiest D.L. (2015). Rpl22 loss impairs the development of B lymphocytes by activating a p53-dependent checkpoint. Journal of immunology (Baltimore, Md. : 1950), 194(1), 200-209.
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2

Splenic B cell expansion assay

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Splenic B cells were purified by negative selection using anti-CD43 magnetic beads (Miltenyi, Bergisch Gladbach, Germany) according to manufacturer’s protocol. Cells were stimulated at 1×106 cells/ml in 96-well plates with 50 μg/ml LPS (Sigma Aldrich, St. Louis, MO), 10 μg/ml polyclonal anti-IgM, F(ab′)2 fragments (Jackson ImmunoResearch, West Grove, PA), or 10 μg/ml anti-CD40 (HM40-3) (BioLegend) + 100 U/ml IL-4 (Peprotech, Rocky Hill, NJ). After 72 hours, expansion was measured by flow cytometry using AccuCount 5.27 μm Blank Particles (Spherotech, Lake Forest, IL) according to manufacturer’s protocol.
Fahl S.P., Harris B., Coffey F, & Wiest D.L. (2015). Rpl22 loss impairs the development of B lymphocytes by activating a p53-dependent checkpoint. Journal of immunology (Baltimore, Md. : 1950), 194(1), 200-209.
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