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Ck8 19 efluor 615

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CK8/19-eFluor®615 is a flow cytometry reagent that can be used to detect and analyze cytokeratin 8 and 19 proteins. It is a monoclonal antibody conjugated to the eFluor®615 fluorescent dye.

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Lab products found in correlation

Paraformaldehyde solution, by Merck Group (2 mentions) Mouse anti human epcam mab, by R&D Systems (2 mentions) Mouse monoclonal anti fc blocker igg, by R&D Systems (2 mentions) Anti pan ck ae1 ae3 efluor 615 mab, by Thermo Fisher Scientific (2 mentions) Anti human epcam efluor 660 mab, by Thermo Fisher Scientific (2 mentions) Anti human vimentin alexa fluor 488 mab, by R&D Systems (2 mentions) Anti human vimentin apc mab, by R&D Systems (2 mentions) Nuclease free water and microtubes, by Thermo Fisher Scientific (2 mentions)

2 protocols using ck8 19 efluor 615

1

Comprehensive Immunophenotyping of Tumor Samples

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COC (6013S-04) was purchased from TOPAS Advanced Polymers (Florence, KY). Chemicals and reagents used in these studies included Micro-90, reagent-grade isopropyl alcohol (IPA), phosphate-buffered saline pH = 7.4 (PBS), 2-(4-morpholino)-ethane sulfonic acid (MES), 7.5% bovine serum albumin (BSA), Triton X-100, paraformaldehyde solution (Sigma-Aldrich, St. Louis, MO), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), N-hydroxysuccinimide (NHS) (Pierce, Rockford, IL), mouse anti-human EpCAM mAb (R&D Systems, clone#158210, Minneapolis, MN), mouse anti-human Fibroblast Activation Protein α (FAPα) mAb (R&D Systems, clone#427819), mouse monoclonal anti-Fc blocker IgG (R&D Systems,), DAPI, anti-CD45-FITC mAb (eBioscience, clone HI30), anti-CK 8 and 19 mAb (CK8/19-eFluor®615, clone#LP3K, BA17), anti-pan-CK-(AE1/AE3) eFluor®615 mAb (eBioscience, San Diego, CA), anti-human EpCAM-eFluor®660 mAb (eBioscience, clone#1B7), anti-human vimentin-Alexa Fluor®488 mAb (clone 280618 (R&D), and anti-human vimentin-APC mAb (R&D Systems, clone#280618). Nuclease-free water and microtubes (Ambion, Foster City, CA) were used for preparation and storage of all samples and reagents.
Witek M.A., Aufforth R.D., Wang H., Kamande J.W., Jackson J.M., Pullagurla S.R., Hupert M.L., Usary J., Wysham W.Z., Hilliard D., Montgomery S., Bae-Jump V., Carey L.A., Gehrig P.A., Milowsky M.I., Perou C.M., Soper J.T., Whang Y.E., Yeh J.J., Martin G, & Soper S.A. (2017). Discrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion molecule. NPJ Precision Oncology, 1, 24.
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2

Immunofluorescence Staining Protocol for CTC Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
COC (6013S-04) was purchased from TOPAS Advanced Polymers (Florence, KY). Chemicals and reagents used in these studies included Micro-90, reagent-grade isopropyl alcohol (IPA), phosphate-buffered saline pH = 7.4 (PBS), 2-(4-morpholino)-ethane sulfonic acid (MES), 7.5% bovine serum albumin (BSA), Triton X-100, paraformaldehyde solution (Sigma-Aldrich, St. Louis, MO), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), N-hydroxysuccinimide (NHS) (Pierce, Rockford, IL), mouse anti-human EpCAM mAb (R&D Systems, clone#158210, Minneapolis, MN), mouse anti-human Fibroblast Activation Protein α (FAPα) mAb (R&D Systems, clone#427819), mouse monoclonal anti-Fc blocker IgG (R&D Systems,), DAPI, anti-CD45-FITC mAb (eBioscience, clone HI30), anti-CK 8 and 19 mAb (CK8/19-eFluor®615, clone#LP3K, BA17), anti-pan-CK-(AE1/AE3) eFluor®615 mAb (eBioscience, San Diego, CA), anti-human EpCAM-eFluor®660 mAb (eBioscience, clone#1B7), anti-human vimentin-Alexa Fluor®488 mAb (clone 280618 (R&D), and anti-human vimentin-APC mAb (R&D Systems, clone#280618). Nuclease-free water and microtubes (Ambion, Foster City, CA) were used for preparation and storage of all samples and reagents.
Witek M.A., Aufforth R.D., Wang H., Kamande J.W., Jackson J.M., Pullagurla S.R., Hupert M.L., Usary J., Wysham W.Z., Hilliard D., Montgomery S., Bae-Jump V., Carey L.A., Gehrig P.A., Milowsky M.I., Perou C.M., Soper J.T., Whang Y.E., Yeh J.J., Martin G, & Soper S.A. (2017). Discrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion molecule. NPJ Precision Oncology, 1, 24.
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