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2 protocols using human serum albumin (hsa)

1

Chiral HPLC Analysis of Enantiomers

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R- and S-APR were purchased from Beijing Mesochem Technology Co. Ltd. (Beijing, China). Human serum albumin was ordered from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan). Methanol (MeOH), ethanol (EtOH), 1-propanol (PROP), 2-propanol (IPA) and acetonitrile (ACN) of gradient grade were purchased from Merck (Darmstadt, Germany). The immobilized Chiral-HSA column (4.0 × 100 mm, 5 μm) was ordered from Chiral Technologies Inc., a subsidiary of Daicel Chemical Industries Ltd. (Tokyo, Japan). Analytical-grade acetic acid, phosphate salts, phosphoric acid, sodium hydroxide and ammonium–acetate were ordered from Sigma-Aldrich, Hungary (Budapest, Hungary). These were used for the preparation of the buffer solution. All reagents were used without further purification. The deionized water was prepared by a Milli-Q Direct 8 Millipore system (Millipore Corporation, Bedford, MA, USA).
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2

Synthesis and Characterization of Mannose-Targeted Liposomes

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Glyceryl monostearate and stearic acid were purchased from Beijing Fengli Jingqiu Commerce and Trade Co., Ltd (Beijing, China). Glyceryl behenate was purchased from Guangzhou Tianrun Pharmaceutical Co., Ltd (Guangzhou, China). Egg yolk lecithin and soybean lecithin were purchased from Shanghai Tywei Co., Ltd (Shanghai, China). Poloxamer 188, Span 85, Tween 80, glycerin, and Brij S10 were obtained from Sigma-Aldrich (ST. Louis, USA). MT and FBS was purchased from Innochem Technology Co., Ltd (Beijing, China). P-nitrophenyl-a-D-mannopyranoside, N-succinimidyl-S-acetylthioacetate (SATA), and phosphatidylethanolamine-PEG-maleimide (DSPE-PEG-MAL) were bought from Shanghai Yiyan Biological Technology Co., Ltd (Shanghai, China). Human serum albumin was purchased from Tokyo Chemical Industry (Shanghai) Development Co., Ltd (Shanghai, China). Phosphorus oxychloride, palladium 10% on carbon, and thiophosgen were bought from Energy Chemical Technology (Shanghai) Co., Ltd (Shanghai, China). Dialysis bags (2500 kDa cutoff) were purchased from Shanghai Yuanye Biological Technology Co., Ltd (Shanghai, China). Ultrafiltration tubes (3 kDa cutoff) were bought from Merck Millipore (Germany). Acetonitrile, methanol, ethanol, and ethyl acetate were of HPLC grade, and other reagents were of analytical grade.
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