RAW cells and HEK 293 cells were grown in Dulbecco’s Modified
Eagle Medium (supplemented with 10% Fetalplex™ Animal
Serum Complex (Gemini Bio-Products), 2 mM L-glutamine and 1%
penicillin/streptomycin). HepG2 cells were grown in DMEM with low
glucose, L-glutamine, sodium pyruvate (Caisson Labs DML28), supplemented with
10% Fetalplex™ Animal Serum Complex and 1%
penicillin/streptomycin. Cells were grown to 90% confluency, washed 1x
with phosphate buffered saline (PBS) and harvested with a cell lifter. Cell
pellets were centrifuged at 1,200 rpm, 3 min and taken up in the desired culture
medium. Whole human blood samples were received from the Stanford Blood Bank and
peripheral mononuclear cells (PMNs) were purified using the
EasySep™ Direct Human Neutrophil Isolation Kit (StemCell
Technologies).
Eagle Medium (supplemented with 10% Fetalplex™ Animal
Serum Complex (Gemini Bio-Products), 2 mM L-glutamine and 1%
penicillin/streptomycin). HepG2 cells were grown in DMEM with low
glucose, L-glutamine, sodium pyruvate (Caisson Labs DML28), supplemented with
10% Fetalplex™ Animal Serum Complex and 1%
penicillin/streptomycin. Cells were grown to 90% confluency, washed 1x
with phosphate buffered saline (PBS) and harvested with a cell lifter. Cell
pellets were centrifuged at 1,200 rpm, 3 min and taken up in the desired culture
medium. Whole human blood samples were received from the Stanford Blood Bank and
peripheral mononuclear cells (PMNs) were purified using the
EasySep™ Direct Human Neutrophil Isolation Kit (StemCell
Technologies).