BMmacs from Dhpsfl/fl and DhpΔmye mice were infected or not with H. pylori for 18 h. Cells were then washed with PBS and methionine-free, cysteine-free DMEM (GIBCO) was added to the cells for 2 h. This medium was then replaced by methionine-free, cysteine-free DMEM supplemented with 50 μM Click-IT homopropargylglycine (Invitrogen) for 4 h. Proteins were extracted using 1% SDS in 50 mM Tris-HCl containing the Protease Inhibitor Cocktail (Set III, Calbiochem) and the Phosphatase Inhibitor Cocktail (Set I, Calbiochem). Proteins (50 μg) were labeled with biotin azide (Invitrogen) using the Click Chemistry Reagents (Invitrogen), precipitated by methanol, resuspended in 50 mM Tris-HCl, and immunoprecipitated using a NOS2 Ab (Millipore) and the PureProteome Protein A Magnetic Bead System (Millipore). The resulting precipitates were used for western blotting using 4plus Streptavidin HRP Label (Biocare Medical).
Nos2 ab
Manufactured by Merck Group
NOS2 Ab is an antibody product manufactured by Merck Group. The antibody specifically targets the inducible nitric oxide synthase (iNOS or NOS2) protein. This enzyme is responsible for the production of nitric oxide, which plays a role in various physiological and pathological processes. The NOS2 Ab product can be used for the detection and quantification of the NOS2 protein in biological samples, such as cell lysates or tissue extracts, through techniques like Western blotting or ELISA.
Automatically generated - may contain errors
Lab products found in correlation
Biotin azide, by Thermo Fisher Scientific (2 mentions)
Click chemistry reagents, by Thermo Fisher Scientific (2 mentions)
Pureproteome protein a magnetic bead system, by Merck Group (2 mentions)
4plus streptavidin hrp label, by Biocare Medical (2 mentions)
Click it homopropargylglycine, by Thermo Fisher Scientific (2 mentions)
2 protocols using nos2 ab
1
Metabolic Labeling of NOS2 Interactome
2
Metabolic Labeling of NOS2 Interactome
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BMmacs from Dhpsfl/fl and DhpΔmye mice were infected or not with H. pylori for 18 h. Cells were then washed with PBS and methionine-free, cysteine-free DMEM (GIBCO) was added to the cells for 2 h. This medium was then replaced by methionine-free, cysteine-free DMEM supplemented with 50 μM Click-IT homopropargylglycine (Invitrogen) for 4 h. Proteins were extracted using 1% SDS in 50 mM Tris-HCl containing the Protease Inhibitor Cocktail (Set III, Calbiochem) and the Phosphatase Inhibitor Cocktail (Set I, Calbiochem). Proteins (50 μg) were labeled with biotin azide (Invitrogen) using the Click Chemistry Reagents (Invitrogen), precipitated by methanol, resuspended in 50 mM Tris-HCl, and immunoprecipitated using a NOS2 Ab (Millipore) and the PureProteome Protein A Magnetic Bead System (Millipore). The resulting precipitates were used for western blotting using 4plus Streptavidin HRP Label (Biocare Medical).
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