Gynostemma pentaphyllum, Trigonella foenum-graecum seeds, Polygala tenuifolia, Ginkgo biloba leaves, Astragalus membranaceus, Radix paeoniae alba and Suanzaorenhehuan were purchased from Harbin Xinheng Traditional Chinese Medicine Materials Ltd. (Harbin, China) and authenticated by Professor Lianjie Su, Heilongjiang University of Chinese Medicine. HP-20 macroporous resin and Sephadex LH-20 were obtained from Soledad Technology Co., Ltd. (Beijing, China). Clorgiline, pargilin, fluoxetine (Flu) were purchased from Sigma (St. Louis, MO, USA). Casein, vanilla acid, 4-aminoantipyrine and horseradish peroxidase were purchased from Aladdin Bio-Chem Technology Co., Ltd. (Shanghai, China).
Fluoxetine flu
Manufactured by Merck Group
Sourced in United States
Fluoxetine (Flu) is a laboratory reagent used in research and analysis. It is a selective serotonin reuptake inhibitor (SSRI) that can be used to study the effects of serotonin regulation in various biological systems. Fluoxetine is a commonly used compound in life science research, but its specific applications and intended uses should not be extrapolated or interpreted in this description.
Automatically generated - may contain errors
4 protocols using fluoxetine flu
1
Multi-herb Extract Preparation and Evaluation
2
Antidepressant Compounds Evaluation
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Cy (98% purity) was provided by Aoke Biology Research Co., Ltd (Beijing, China). Fluoxetine (Flu) was obtained from Sigma-Aldrich (St. Louis, USA). 3-TYP was purchased from MedKoo Biosciences, Inc (Chapel Hill, USA). Both α-cyperone (0.5 mg/ml; 1 mg/ml) and Fluoxetine (2 mg/ml) were dissolved in 0.05% DMSO and intragastrically administrated at a volume of 10 ml/kg.
3
Isoliquiritin and Fluoxetine Comparison
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Isoliquiritin (purity ≥ 98%) was obtained from Chengdu Must Bio-Technology Co., Ltd (Sichuan, China). Fluoxetine (Flu) was purchased from Sigma-Aldrich (St. Louis, USA). Flu (20 mg/kg) was selected as positive control based on previous studies [17 (link), 18 (link)].
4
Standardized Evaluation of H. gordonii Extract
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In this study, a commercial dry purified extract of H. gordonii was
used, legally imported from Naturalmedis (UK). This product is manufactured in
accordance with the requirements of the Medicines and Healthcare Products Regulatory
Agency in the United Kingdom. The active ingredient content (P57) is guaranteed
through regular analyses performed by the company's Laboratory for Quality Control.
Also, the material was shown to be free of microbiological contamination and was at a
dilution of 20:1. The purchase of the substance was made for research purposes. For
the use of the drug in our experiments, H. gordonii was emulsified
in 0.3% Tween 20 (Sigma, USA) and diluted in distilled water. The extract was
administered orally to mice, at doses of 25 and 50 mg/kg in a single dose or for 15
consecutive days.
The additional drugs used is this study were: 10 mg/kg imipramine (IMI; Geisy,
Brazil), 1 mg/kg prazosin (PZS; Sigma), 1 mg/kg yohimbine (YOHI; Sigma), 0.05 mg/kg
SCH23390 (Sigma), 50 mg/kg sulpiride (Sigma), 100 mg/kg
p-chlorophenylalanine methyl ester (PCPA; Sigma), 0.5 mg/kg NAN-190
{1-(2-methoxyphenyl)-4-[4-(2-phthalimido)butyl]piperazine, Sigma}, 1 mg/kg
ondansetron (Sigma), 1 mg/kg ritanserin (Sigma), and 35 mg/kg fluoxetine (FLU;
Sigma). All drugs were diluted in distilled water and administered by the
intraperitoneal (ip) route.
used, legally imported from Naturalmedis (UK). This product is manufactured in
accordance with the requirements of the Medicines and Healthcare Products Regulatory
Agency in the United Kingdom. The active ingredient content (P57) is guaranteed
through regular analyses performed by the company's Laboratory for Quality Control.
Also, the material was shown to be free of microbiological contamination and was at a
dilution of 20:1. The purchase of the substance was made for research purposes. For
the use of the drug in our experiments, H. gordonii was emulsified
in 0.3% Tween 20 (Sigma, USA) and diluted in distilled water. The extract was
administered orally to mice, at doses of 25 and 50 mg/kg in a single dose or for 15
consecutive days.
The additional drugs used is this study were: 10 mg/kg imipramine (IMI; Geisy,
Brazil), 1 mg/kg prazosin (PZS; Sigma), 1 mg/kg yohimbine (YOHI; Sigma), 0.05 mg/kg
SCH23390 (Sigma), 50 mg/kg sulpiride (Sigma), 100 mg/kg
p-chlorophenylalanine methyl ester (PCPA; Sigma), 0.5 mg/kg NAN-190
{1-(2-methoxyphenyl)-4-[4-(2-phthalimido)butyl]piperazine, Sigma}, 1 mg/kg
ondansetron (Sigma), 1 mg/kg ritanserin (Sigma), and 35 mg/kg fluoxetine (FLU;
Sigma). All drugs were diluted in distilled water and administered by the
intraperitoneal (ip) route.
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