Following indicated treatment, cells were harvested and washed with PBS. Intracellular FFA levels were measured using a FFA Quantification Colorimetric kit (BioVision) according to the manufacturer’s protocol. The samples were measured against a standard of varying concentrations of palmitic acid (PA) and the optical density was measured at 570 nm in a Tecan plate reader. The levels of FFA were calculated using the slope of the standard curve.
Ffa quantification colorimetric kit
Manufactured by Abcam
Sourced in United States
The FFA quantification colorimetric kit is a laboratory tool used to measure the concentration of free fatty acids (FFAs) in a sample. The kit provides a simple and reliable method for quantifying FFAs through a colorimetric reaction.
Automatically generated - may contain errors
3 protocols using ffa quantification colorimetric kit
1
Quantification of Intracellular Free Fatty Acids
2
Serum biomarker quantification protocol
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Serum SC levels were determined by EnzyChrom
SC assay Kit (#ESNT-100, BioAssay Systems, Hayward, CA, USA). Serum
cytokine IL-6 and TNF-a levels were measured by commercial mouse ELISA
kits (BioLegend, Inc., San Diego, CA, USA). Serum free fatty acid
(FFA) was measured using a FFA quantification colorimetric kit (Biovision,
Milpitas, CA, USA).
SC assay Kit (#ESNT-100, BioAssay Systems, Hayward, CA, USA). Serum
cytokine IL-6 and TNF-a levels were measured by commercial mouse ELISA
kits (BioLegend, Inc., San Diego, CA, USA). Serum free fatty acid
(FFA) was measured using a FFA quantification colorimetric kit (Biovision,
Milpitas, CA, USA).
3
Quantifying Hepatic Lipid Levels
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According to the manufacturer’s protocol, lipid was extracted from the liver tissue with a lipid extraction kit (BioVision, Inc., Milpitas, CA, USA). The extracted lipid was then suspended in 50 µL of lipid suspension buffer and sonicated for 15–20 min at 37 °C before quantifying the level of FFA (FFA quantification colorimetric kit, BioVision Inc., Milpitas, CA, USA) and TG (Triglyceride quantification colorimetric kit, BioVision Inc., Milpitas, CA, USA) by colorimetric assay. Hepatic FFA and TG levels were expressed as nmol/mg of tissue.
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