Anti cd81 antibody

Unless otherwise specified all reagents are from Sigma-Aldrich. Opti-MEM, FluoroBrite™ DMEM, Lipofectamine® transfection reagent, DAPI, CellTrace™ CFSE Cell Proliferation Kit and CellTracker™ Orange Dye are from Invitrogen™ Life Technologies. siRNAs for Gal-1, RNase-free DNase set are from Qiagen. Sh-RNA vectors are purchased from OriGene. RNA Nano Chip kit is from Agilent. PrimeScript RT reagent kit, ProteaseMAX™ Surfactant, Sequencing Grade Modified Trypsin are from Promega. Polyvinylidene difluoride (PVDF) membrane is from Millipore. Transwells are purchased from Euroclone. Anti-Gal-1 antibody is from Cell Signaling, anti-β-actin and anti-Integrin-β1 antibodies are from Santa Cruz Biotechnology, anti-CD81 antibody is from BD Biosciences. The secondary antibodies enzyme horseradish peroxidase (HRP)–conjugated are from Santa Cruz Biotechnology. All materials for SDS-PAGE and Clarity™ Western ECL substrate are from Biorad. Culture-inserts (Dish 35 mm, high) are from ibidi®.

Antibodies were purchased from the following sources: anti-CD81 antibody was from BD Pharmingen, mouse anti-actin antibody was from Sigma-Aldrich, and ISG15 and MxA antibodies were from Santa Cruz. HCV core, NS3, NS5A antibodies have been described elsewhere^{14 (link)}. HCV E2 antibody was a gift from Dr. Jean Dubuisson (Institut Pasteur de Lille). Tylophorine was purchased from Alexis Biochemicals. O859585, T298875, and Sofosbuvir were purchased from Toronto Research Chemicals and MedChemexpress.