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Bond rx automated stainer

Manufactured by Leica Biosystems
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Sourced in Germany

The Bond RX Automated Stainer is a compact, reliable, and user-friendly equipment designed for automated staining of histology and cytology samples. It offers consistent and reproducible slide preparation, streamlining the staining process in the laboratory.

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Lab products found in correlation

Bond epitope retrieval solution 2, by Leica Biosystems (1 mentions) Bond polymer refine detection, by Leica Biosystems (1 mentions) Aperio cs2, by Leica Biosystems (1 mentions) Vectra polaris slide scanner, by Akoya Biosciences (1 mentions) Envision dual link, by Agilent Technologies (1 mentions) Dab enhancer, by Leica Biosystems (1 mentions)

3 protocols using bond rx automated stainer

1

Immunohistochemical Analysis of γ-H2AX in Tumor Tissue

Cited 2 times
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Tumor tissue was fixed in 10% neutral buffered formalin and embedded in paraffin, before being sectioned (4 μM thick) and stained using the Bond RX Automated Stainer (Leica Biosystems). Heat induced antigen retrieval was performed at pH 9 (Bond Epitope Retrieval solution 2, Leica Biosystems), 100 °C for 30 min, before Υ-H2AX antibody incubation (1:500 dilution, Cell Signaling, Clone 20E3) for 60 min. Detection was performed with diaminobenzidine (Bond Polymer Refine Detection, Leica Biosystems) and slides were counterstained with haematoxylin. Slides were imaged using a slide scanner (AperioCS2, Leica Biosystems), and data were analyzed using QuPath52 (link) as described53 (link).
Aziz D., Portman N., Fernandez K.J., Lee C., Alexandrou S., Llop-Guevara A., Phan Z., Yong A., Wilkinson A., Sergio C.M., Ferraro D., Etemadmoghadam D., Bowtell D.D., Serra V., Waring P., Lim E, & Caldon C.E. (2021). Synergistic targeting of BRCA1 mutated breast cancers with PARP and CDK2 inhibition. NPJ Breast Cancer, 7, 111.
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2

Ki-67 Analysis of NCI-237-R PDX Tumors

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NCI-237-RPDX tumor fragments from vehicle- and NCGC00420737-treated animals were fixed in 10% neutral-buffered formalin and incubated at 4°C for 24 hours with gentle shaking. Fixed tumors were submitted to the UT Southwestern Histo Pathology Core for paraffin embedding and sectioning. Hematoxylin- and eosin-staining was performed by the UT Southwestern Histo Pathology Core. Ki-67 immunohistochemical staining was performed by the UT Southwestern Simmons Comprehensive Cancer Center Tissue Management Shared Resource using a BOND-RX Automated Stainer (Leica Biosystems) and human-specific anti-Ki-67 antibody (Cell Signaling Technology #9027, RRID: AB_2636984, 1:400 dilution). Whole-slide scans were captured at 40X magnification using a Vectra Polaris slide scanner (Akoya Biosciences) and images were generated using Phenochart™ 1.1.0 software (Akoya Biosciences).
Salzmann M., Pervushin K., Wider G., Senn H, & Wüthrich K. (1998). TROSY in triple-resonance experiments: New perspectives for sequential NMR assignment of large proteins. Proceedings of the National Academy of Sciences of the United States of America, 95(23), 13585-13590.
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3

Immunohistochemical Staining of p53β and CA9

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For immunohistochemical (IHC) staining, 4‐μm sections from paraffin‐embedded tissues were used. The KJC8 antibody towards p53β was generously provided by the Bourdon Laboratory (University of Dundee, Ninewells Hospital and Medical School, Dundee, UK) and was detected using EnVision Dual Link (Dako, Glostrup, Denmark) and diaminobenzidine chromogen (DAB), with DAB enhancer (Leica Biosystems, Wetzlar, Germany). The CA9 antibody (MRQ‐54; Cell Marque, Rocklin, CA, USA) was used to detect CA9‐positive cells using an automated IHC method (BOND RX automated stainer; Leica Biosystems, Wetzlar, Germany).
Kazantseva M., Eiholzer R.A., Mehta S., Taha A., Bowie S., Roth I., Zhou J., Joruiz S.M., Royds J.A., Hung N.A., Slatter T.L, & Braithwaite A.W. (2018). Elevation of the TP53 isoform Δ133p53β in glioblastomas: an alternative to mutant p53 in promoting tumor development. The Journal of Pathology, 246(1), 77-88.
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