Its g 100x

Manufactured by Thermo Fisher Scientific

Sourced in United States, Austria

The ITS-G 100X is a laboratory instrument designed for optical analysis. It features a 100X magnification capability to provide detailed visual inspection of samples. The core function of the ITS-G 100X is to enable high-resolution microscopic examination of specimens.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

ITS-G (28 citations) ITS-100x (2 citations)

6 protocols using its g 100x

Decellularized Extracellular Matrix Effects on Cardiac Explants

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hearts harvested from day 1 mice were washed in cold 1 x PBS. Atrial was removed and ventricle was cut to approximately 1mm³ pieces using surgical scissors. Two microliters of adult or fetal dECM were injected into explants using 10μl Hamilton syringes with 23G needles before plating on 48-well plates coated with 1.5mg/ml collagen I (Corning) gel. Explants were incubated at 37°C for 2h to induce dECM polymerization and explant-collagen gel attachment. Explants were cultured in explant culture media [1% fetal bovine serum, 2mM L-glutamine, 1 x insulin-transferrin-selenium (Gibco, ITS-G 100X) in M199] for 6 days with 10μM BrdU labeling for 3 days before fixation. Paraformaldehyde (4%) was used for fixation. In separate experiments, explants were cultured in explant culture media supplemented with 5mM ribose or 0.2mM BAPN for 6 days from plating in combination with dECM treatment conditions. Atomic force microscopy was used to measure the elastic modulus of decellularized explants [13 (link)], [32 (link)].
In order to regulate cytoskeleton polymerization, explants were cultured in explant culture media supplemented with Jasplakinolide (Cayman), Latrunculin A (Cayman), Y27632 (Chemdea), PF573228 (Selleck) for 3 days; no BrdU labeling applied.

Wang X., Pierre V., Liu C., Senapati S., Park P.S, & Senyo S.E. (2021). Exogenous extracellular matrix proteins decrease cardiac fibroblast activation in stiffening microenvironment through CAPG. Journal of molecular and cellular cardiology, 159, 105-119.

+ Open protocol

+ Expand

Podocyte Differentiation and Stress Assays

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Control and patient-derived iPSC were differentiated into podocytes according to the protocol we developed previously [9 (link)]. From day 13 to day 18, iPSC-derived podocytes were cultured in RPMI 1640 medium (Gibco, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS), ITS 1X (insulin–transferrin–selenium; ITS-G, 100X; Gibco), and 50 U/mL penicillin plus 0.05 mg/mL streptomycin (Gibco).
To evaluate cytoskeleton rearrangement, iPSC-derived podocytes at day 13 of diffe-rentiation were exposed to angiotensin II (Ang II, 500 nM, Sigma-Aldrich, St. Louis, MO, USA) for 24 h in DMEM-F12 + GlutaMAX (Gibco) in serum-free conditions.
To analyze susceptibility to apoptosis, at day 13 iPSC-derived podocytes were exposed to the puromycin aminonucleoside (PAN, 100 μg/mL, Sigma-Aldrich) in DMEM-F12 + GlutaMAX supplemented with 1% FBS.

Longaretti L., Trionfini P., Brizi V., Xinaris C., Mele C., Breno M., Romano E., Giampietro R., Remuzzi G., Benigni A, & Tomasoni S. (2021). Unravelling the Role of PAX2 Mutation in Human Focal Segmental Glomerulosclerosis. Biomedicines, 9(12), 1808.

+ Open protocol

+ Expand

Ovarian Cancer Cell Line Cultivation

Check if the same lab product or an alternative is used in the 5 most similar protocols

For the purpose of this study two commercially available human ovarian epithelial adenocarcinoma cell lines were used (SKOV-3 and OVCAR-3). Both cell types were acquired from the American Type Culture Collection (ATCC, Manassas, Virginia, USA) and cultivated according to the specification of the supplier in base media supplemented with heat-inactivated fetal calf/bovine serum (FCS, Gibco, Thermo Fisher Scientific, Austria) and maintained in humidified incubators (37°C and 5% CO₂). SKOV-3 cells were cultivated in McCoy’s 5a Medium Modified (Gibco, Thermo Fisher Scientific, Austria) with 10% (v/v) FCS and 1% (v/v) penicillin-streptomycin (P/S, Sigma-Aldrich, Austria), and OVCAR-3 cells were cultivated in RPMI-1640 Medium (Gibco, Thermo Fisher Scientific, Austria) supplemented with 20% (v/v) FCS, 1% (v/v) P/S and 1% (w/v) insulin-transferrin-selenium (ITS-G, 100X, Gibco, Thermo Fisher Scientific). Cell number was determined with a MOXI Z Mini Automated Cell Counter with the corresponding MOXI Z Type M Cassettes (ORFLO Technologies, USA) and experiments were performed with cells reaching a confluency of 80%.

Bileck A., Bortel P., Kriz M., Janker L., Kiss E., Gerner C, & Del Favero G. (2022). Inward Outward Signaling in Ovarian Cancer: Morpho-Phospho-Proteomic Profiling Upon Application of Hypoxia and Shear Stress Characterizes the Adaptive Plasticity of OVCAR-3 and SKOV-3 Cells. Frontiers in Oncology, 11, 746411.

+ Open protocol

+ Expand

Cardiac Explant Culture and Cytoskeleton Modulation

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cell and explant culture media contain 1U/ml P/S. Primary cardiomyocytes plated with ECM were cultured in DMEM supplemented with 15% fetal bovine serum (FBS) at 37°C overnight. Cells were then cultured in DMEM, followed by culturing in DMEM containing 10μM BrdU. Cells were fixed in 4% paraformaldehyde (PFA).
Cardiac explants were cultured in M199 supplemented with 1% FBS, 1:100 diluted insulin-transferrin-selenium (Gibco, ITS-G 100X), and 2mM L-glutamine [explant culture media] for 3 days [44 (link)]. Explants were then cultured in explant culture media with 10μM BrdU for cell cycle labeling for 3 days. Explants were fixed in 4% PFA. In separate experiments, explants were cultured in explant culture media supplemented with 5mM ribose or 0.2mM BAPN for 6 days from plating in combination with ECM treatment conditions. In order to tune cytoskeleton, explants were cultured in explant culture media supplemented with Jasplakinolide (Cayman), Latrunculin A (Cayman), Y27632 (Chemdea), PF573228 (Selleck) for 3 days; no BrdU labeling applied.

Wang X., Senapati S., Akinbote A., Gnanasambandam B., Park P.S, & Senyo S.E. (2020). Microenvironment stiffness requires decellularized cardiac extracellular matrix to promote heart regeneration in the neonatal mouse heart. Acta biomaterialia, 113, 380-392.

+ Open protocol

+ Expand

Culturing DolKT1 Cells for Experiments

Check if the same lab product or an alternative is used in the 5 most similar protocols

DolKT1 cells were cultured in Dulbecco’s modified eagle medium (DMEM) (Cat# 11885084; Gibco, Grand Island, NY) supplemented with 10% FBS (Nichirei Biosciences, Tokyo, Japan), 1% insulin-transferrin-selenium (ITS-G 100X, # 41400045, Thermo Fisher, Waltham, MA), 50 U streptomycin/mL and 50 mg penicillin/mL (Life Technologies, Van Allen Way Carlsbad, CA). The cells were grown at 37 °C in a humidified atmosphere with 5% CO₂. We tested for mycoplasma on a regular basis. The cells were passaged at 90% confluency and exposed to different experimental conditions. The culture medium was changed with 1% FBS (if not mentioned separately) for 18 h before the experiments. All experiments were performed between passages 24 and 30.

Jahan N., Ohsaki H., Kaneko K., Rahman A., Nishiyama T., Koizumi M., Yamanaka S., Kitada K., Sugiura Y., Matsui K., Yokoo T., Hamano T., Kuro-o M., Itou T., Suzuki M., Ueda K, & Nishiyama A. (2023). Possible contribution of phosphate to the pathogenesis of chronic kidney disease in dolphins. Scientific Reports, 13, 5161.

+ Open protocol

+ Expand

Cell Line Cultivation and Starvation

Check if the same lab product or an alternative is used in the 5 most similar protocols

All cell lines were cultured at 37°C with 5% CO₂. MDA-MB-231, Huh7, HEK293A cell lines were cultured in DMEM (Life Technologies #11965092) supplemented with 10% FBS. MCF10A were cultured in DMEM/F12 (Life Technologies #11330032) containing 5% horse serum, 20 ng/mL EGF, 0.5 μg/mL hydrocortisone, 10 μg/mL insulin, 100 ng/mL cholera toxin, and 50 μg/mL penicillin/streptomycin (P/S). Primary mouse hepatocytes were cultured in William’s E medium (Life Technologies #12551032) containing 10% FBS and 1% P/S. AML12 cells were cultured in DMEM: F12 medium (ATCC #30–2006) supplemented with 10% FBS, Insulin-Transferrin-Selenium (ITS-G) (100X) (Thermo Fisher Scientific #41400045), and 40 ng/mL dexamethasone. For signaling assays, cells were washed 1x in PBS and placed in starvation media (-FBS) for 2–16 h depending upon cell line.

Lin T.Y., Ramsamooj S., Perrier T., Liberatore K., Lantier L., Vasan N., Karukurichi K., Hwang S.K., Kesicki E.A., Kastenhuber E.R., Wiederhold T., Yaron T.M., Huntsman E.M., Zhu M., Ma Y., Paddock M.N., Zhang G., Hopkins B.D., McGuinness O., Schwartz R.E., Ersoy B.A., Cantley L.C., Johnson J.L, & Goncalves M.D. (2023). Epinephrine inhibits PI3Kα via the Hippo kinases. Cell reports, 42(12), 113535.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!