Mouse il 21r human fc

Manufactured by R&D Systems

Mouse IL-21R-human Fc is a recombinant protein that consists of the extracellular domain of the mouse interleukin-21 receptor (IL-21R) fused to the Fc region of human immunoglobulin G (IgG). It is designed for use in various in vitro and in vivo applications.

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Lab products found in correlation

Anti human fc pe, by BD (2 mentions)

Spelling variants of this product

Mouse IL-21R–Fc (2 citations) Recombinant mouse IL-21R human FC chimera (2 citations)

2 protocols using mouse il 21r human fc

MHC-Restricted Peptide Stimulation and Intracellular IL-21 Staining

Cited 1 time

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For MHC class-I-restricted GP_33–41 peptide (2 μg/ml) or MHC class-II restricted GP_67–77 (5 μg/ml) stimulation and staining were carried out in the presence of Brefeldin A (BFA) as we have previously described (21 (link)). For intracellular IL-21 staining, cells were permeabilized with saponin and incubated with 1:25 dilution of mouse IL-21R-human Fc (R&D Systems) for 30 minutes at 4°C, washed twice and stained with 1:200 anti-human Fc-PE (BD Pharmingen).

Wong K.A., Harker J.A., Dolgoter A., Marooki N, & Zuniga E.I. (2019). T cell-intrinsic IL6R signaling is required for optimal ICOS expression and viral control during chronic infection. Journal of immunology (Baltimore, Md. : 1950), 203(6), 1509-1520.

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Intracellular IL-21 Staining Protocol

Cited 1 time

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For MHC class-I-restricted GP_33–41 peptide (2 µg/ml) or MHC class-II restricted GP_67–77 (5 µg/ml) stimulation and staining were carried out as we have previously described (31 (link)). For polyclonal stimulation we used PMA (10 ng/ml) and ionomicyn (0.5 µg/ml) in place of peptide. For intracellular IL-21 staining, cells were permeabilized with saponin and incubated with 1:25 dilution of mouse IL-21R-human Fc (R&D Systems) for 30 minutes at 4°C, washed twice and stained with 1:200 anti-human Fc-PE (BD Pharmingen).

Harker J.A., Wong K.A., Dolgoter A, & Zuniga E.I. (2015). Cell-intrinsic gp130 Signaling on CD4+ T cells Shapes Long-lasting Antiviral Immunity. Journal of immunology (Baltimore, Md. : 1950), 195(3), 1071-1081.

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