Ad ctrl

Manufactured by Hanbio Biotechnology

Sourced in China

The Ad.ctrl is a laboratory equipment designed for the purpose of analysis and control. It provides precise and reliable measurement capabilities for various applications within the biotechnology field.

Automatically generated - may contain errors

Lab products found in correlation

Ultra fine insulin syringe, by BD (1 mentions) Oe nc, by Tsingke (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Ad shctrl, by Genechem (1 mentions)

2 protocols using ad ctrl

Bile Duct Ligation and BMSC Therapy

Cited 1 time

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Adult C57BL/6J male mice (8 weeks old) and aged mice (13–16 months old) were housed in a pathogen-free animal facility under a standard 12-hour light/dark cycle. Mice were given standard rodent chow and water ad libitum. Adult C57BL/6J male mice were divided randomly into four groups, and bile duct ligation in the mice was performed as previously described.³⁹ (link) BMSCs obtained from C57BL/6J male mice (6 weeks old) were isolated and cultured according to an established protocol.⁴⁰ (link) On post-operative day 23, 1 × 10⁶ of Lenti.ctrl and Lenti.sg126 preconditioned BMSCs were injected via the caudal vein. Ad.sg126 mice and Ad.ctrl mice (C57BL/6J background) were generated using recombination Ad.sg126 or Ad.ctrl (Hanbio, Shanghai, China). Viral particles (1.0 × 10¹⁰) were reconstituted in 100 μL injected intravenously through the tail vein with BD Ultra-Fine Insulin Syringes. All procedures followed the Huazhong Agricultural University Guidelines for the Care and Use of Laboratory Animals.

Yan Y., Qin D., Hu B., Zhang C., Liu S., Wu D., Huang W., Huang X., Wang L., Chen X, & Zhang L. (2019). Deletion of miR-126a Promotes Hepatic Aging and Inflammation in a Mouse Model of Cholestasis. Molecular Therapy. Nucleic Acids, 16, 494-504.

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Adenoviral Overexpression and Knockdown of Slc40a1

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Ad-Slc40a1 and Ad-Ctrl constructs were generated by Hanbio Biotechnology (China) using adenoviral vectors carrying Slc40a1. Ad-shSlc40a1 and Ad-shCtrl were provided by GeneChem Technology (Shanghai, China), in the form of adenoviral vectors carrying shRNA-Slc40a1.Protein expression was observed 48 hours after viral infection. The plasmid containing Steap4 and the negative control plasmid (OE-NC) were created and produced by Tsingke Biotech (China). Cardiomyocytes were transfected with the plasmid using Lipofectamine 3000 from Invitrogen (USA). Protein expression was measured 72 hours after transfection. The shRNA target sequences are shown in Table S3.

Feng R., Wang D., Li T., Liu X., Peng T., Liu M., Ren G., Xu H., Luo H., Lu D., Qi B., Zhang M, & Li Y. (2024). Elevated SLC40A1 impairs cardiac function and exacerbates mitochondrial dysfunction, oxidative stress, and apoptosis in ischemic myocardia. International Journal of Biological Sciences, 20(2), 414-432.

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