Prism 7.2 graph pad

Patient’s descriptive analysis was generated, and their differences were investigated using Student t-test for quantitative data; for qualitative data, we used either Fisher’s exact test or chi-square test. To compare overall survival (OS) between groups, the cumulative survival proportions were calculated using the product limit method of Kaplan–Meier, and differences were evaluated using the log-rank test. Only variables that achieved statistical significance in the univariate analysis were subsequently evaluated in the multivariate analysis using Cox’s proportional hazard regression model. A p value of less than 0.05 was considered statistically significant. All statistical analyses were performed using the MedCalc Statistical Software version 14.8.1 (MedCalc Software bvba, Ostend, Belgium) and PRISM 7.2 Graph PAD.

Patients’ descriptive analysis was generated, and their differences were investigated using Student’s t-test for quantitative data; normality test according to D’Agostino-Pearson was performed, and when not passed, quantitative data were compared using the Mann–Whitney test. For qualitative data, we used either the Fisher’s exact test or the Chi-square test. Overall survival analyses were carried out with the Kaplan–Meier method, and differences were evaluated using log-rank test. Only variables that achieved statistical significance in the univariate analysis were subsequently evaluated in the multivariate analysis using Cox’s proportional hazard regression model. ROC (receiver operating characteristic curve and Area Under the Curve) curves and AUC have also been calculated with the help of statistical software. A p-value of less than 0.05 was considered statistically significant. All statistical analyses were performed using the MedCalc Statistical Software version 14.8.1 (MedCalc Software, Ostend, Belgium), Prism 7.2 GraphPad, and SPSS, IBM version 23.
In vitro statistical analysis was carried out using the ANOVA followed by the nonparametric Mann–Whitney U-test or a two-tailed unpaired Student’s t-test comparisons (* p < 0.05) using the Prism 6.0 software (GraphPad San Diego, CA, USA).