Anti flag tag mouse monoclonal antibody

The following antibodies were used in this study: anti-GFP rabbit monoclonal antibody (Beyotime, AF1483), anti-RPA32 rabbit monoclonal antibody (Beyotime, AG3115), anti-FLAG tag mouse monoclonal antibody (Beyotime, AF2852), anti-β-actin monoclonal antibody (Proteintech, 66009–1-Ig), goat anti-rabbit polyclonal secondary antibody IgG (Beyotime, A0208), goat anti-mouse polyclonal secondary antibody IgG (Beyotime, A0216), Alexa 568 goat anti-rabbit polyclonal IgG (H+L) cross-adsorbed secondary antibody (Thermo, A_11036), Alexa 488 goat anti-mouse polyclonal IgG (H+L) cross-adsorbed secondary antibody (Thermo, A32723).

The canine promoter reporter plasmid pIFNβ-Luc was constructed according to a previous study [25 (link)]; a pISRE-Luc and pRL-TK were purchased from Beyotime (Shanghai, China) and Promega (USA), respectively. Poly(I:C) (Sigma, USA) at a final concentration of 2 g/mL was the control for promoter activation experiments. Lipo8000 (Beyotime, China) was used for plasmid transfection according to the manufacturer’s instructions. Anti-GAPDH, anti-Flag-tag mouse monoclonal antibody and anti-IRF1 rabbit monoclonal antibody (Beyotime, China) were used in IFA and western blot. Small interfering RNA (siRNA) (+) against CaIRF1 sequence, 5′-GCACCAGUGACCUCUACAGTT-3′, the siRNA (−) sequence, 5′-CUGUAGAGGUCACUGGUGCTT-3′, and the negative control (NC) siRNA were purchased from Tsingke Biotech (Beijing, China). The primers used in the study were synthesized by Sangon Biotech (Shanghai, China).