CHO cells were cultured in F12 medium (Wako, Japan) supplemented with 10 % foetal bovine serum (FBS) (PAA Laboratories GmbH, Australia) and 1 % penicillin/streptomycin (Gibco, USA). Mouse A9 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco) supplemented with 10 % FBS and 1 % penicillin/streptomycin. TT2 and TT2F mES cells were hybrids of C57BL/6 and CBA strains (a gift from Dr. S. Aizawa, RIKEN, Japan). TT2 and TT2F mES cells and MI-MAC mES cell lines were cultured in KnockOut DMEM (Gibco) supplemented with 5 % FBS, 15 % KNOCKOUT SR (KSR; Gibco), 1× minimum essential medium non-essential amino acids (Gibco), 1× GlutaMAX™-1 (Gibco), 1× nucleosides (Millipore, Germany), 55 µM 2-mercaptoethanol (Gibco), 2.0 × 106 units/mL ESGRO mLIF medium supplement (Millipore), and 1 % penicillin/streptomycin.
Esgro mlif medium supplement
Manufactured by Merck Group
Sourced in Germany
ESGRO mLIF medium supplement is a laboratory product manufactured by Merck Group. It is used as a medium supplement for the culture of mouse embryonic stem cells. The primary function of this product is to maintain the self-renewal and pluripotency of mouse embryonic stem cells in vitro.
Automatically generated - may contain errors
Lab products found in correlation
2 mercaptoethanol, by Thermo Fisher Scientific (2 mentions)
Nucleoside, by Merck Group (1 mentions)
Knockout dmem, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
F12 medium, by Fujifilm (1 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions)
Glutamax 1, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Foetal bovine serum (fbs), by GE Healthcare (1 mentions)
Aphidicolin, by Merck Group (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Doxycycline dox, by Merck Group (1 mentions)
Mem nea, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Ultraglutamine, by Lonza (1 mentions)
2 protocols using esgro mlif medium supplement
1
Culturing Cell Lines for Stem Cell Research
2
Conditional CDC6 Overexpression in mESCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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TetO-CDC6 mESCs derived from TetO-CDC6 mice (35 (link)) were cultured on 0.1% gelatin-coated plates in Dulbecco's modified Eagle's medium (DMEM) with Ultraglutamine 1 and 4.5 g/l glucose (Lonza) supplemented with 15% FBS (Sigma), 50 U/ml penicillin–50 mg/ml streptomycin (Invitrogen), minimum essential medium non-essential aminoacids (MEM NEA; Invitrogen), 100 μM 2-mercaptoethanol (Invitrogen) and 103 U/ml ESGRO mLIF medium supplement (Millipore). To induce CDC6 overexpression, 1 μg/ml doxycycline (dox, Sigma) was added to the medium for 30 h. When indicated, mESCs were treated with 0.5 μM aphidicolin (Sigma-Aldrich) for 2.5 h to induce mild RS.
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