Beaker with magnetic stirrer (Ultraturrax IKA, Wilmington, NC, USA), analytical balance, drinking trough and cages of acrylic, Mettler Toledo DSC882e, scanning electron microscope (SEM JSM 6010LA, JEOL, Dearborn Road, Peabody, MA, USA), Spectra-Pro membrane dialysis tubing 45–50 KDa (Spectra/Por, Miami, FL, USA), Labwit ZWY-103D shaker-incubator, ultra-centrifuge, HALO DB-20 UV–Vis double-beam spectrophotometer (TechComp, Mexico City, Mexico).
Jsm 6010la sem
Manufactured by JEOL
Sourced in Japan, United States
The JSM-6010LA is a scanning electron microscope (SEM) manufactured by JEOL. As an SEM, its core function is to produce high-resolution images of a sample's surface by scanning it with a focused beam of electrons. The JSM-6010LA provides detailed information about the sample's topography, composition, and other characteristics.
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8 protocols using jsm 6010la sem
1
Membrane Dialysis and Material Characterization
2
Nanoformulation Development and Characterization
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Beaker with magnetic stirrer (Ultraturrax), analytical balance, distillation equipment, drinking trough and cages of acrylic, Malvern Z Sizer-Lab, Mettler Toledo DSC882e, scanning electron microscope (SEM JSM 6010LA, JEOL, Dearborn Road Peabody, MA, USA), Spectra-Pro membrane dialysis tubing 45–50 KDa (Spectra/Por, FL, USA), Labwit ZWY-103D shaker-incubator, ultra-centrifuge, HALO DB-20 UV–Vis double beam spectrophotometer (TechComp, Mexico city, Mexico), and an Accu-chek™ active digital glucometer (Roche, Mexico city, Mexico).
3
Ultrastructural Imaging of Liver Sinusoidal Endothelial Cells
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Freshly isolated LSEC from mice were plated at 1 x 106/ml on collagen-coated cover slips in a 24 well plate and incubated at 37C for 8 hours. Cells were fixed in 2.5% glutaraldehyde for 2 hours. Following a cacodylate buffer rinse, the cells were secondary fixed in 1% OsO4, then rinsed again in buffer and dehydrated in EtOH solutions, 50%, 70%, 90%, 100% (X2), for 15min each. Following dehydration, the cells were critical point dried in a LEICA EM CPD 300 using 100% EtOH as the transition liquid. The coverslips were mounted on SEM stubs with double sided carbon tape and sputter coated with gold/palladium for 30 seconds using the Leica EM ACE200. The samples were then imaged using a JEOL JSM-6010LA SEM operating at 10kV.
4
Identifying Metal Particles in HEPA Filters
5
Surface Morphology of Waste Lipstick-Doped Bitumen
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The direct effect imposed by the usage of various doses of waste lipstick (e.g., 5, 10, and 15 wt.% WLS) on the elemental composition and surface morphology/topography of fresh-plain base AP-5 bitumen was examined in detail using JSM-6010LA SEM (JSM-6010LA, JEOL Ltd., Tokyo, Japan) coupled with energy-dispersive X-ray spectroscopy (EDXS). To promote their electrical conductivities, the different asphalt samples, including WLS, were totally submerged in a liquid nitrogen (LN2, −80 °C), before being sprinkled with a thin coating of gold (size of approximately 10 nm) using a X sputter coater (Sputter Coater Model 108auto C3783, Cressington Scientific Instruments, England, UK). Finally, the following circumstances were adopted to collect the SEM micrographs: magnification (×3000), beam current 5 nA, working distance 10 nm, and accelerating voltage (5 kV, 15 kV).
6
Scanning Electron Microscopy Analysis
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AVHX digital microscope (Keyence Corp.) was used. Scanning electron microscopy (SEM). A JEOL JSM-6010LA. SEM equipped with an energy-dispersive spectroscopy (EDS) instrument was used.
7
Fibrin Gel Morphology Analysis
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Analysis of gel morphology was assessed using 200 uL fibrin gels in a 48-well tissue culture plate (Corning). After gelation, gels were hydrated in PBS for one hour, then dehydrated in ethanol (50%, 75%, 90%, and 100% ethanol for one hour each). Samples were attached to SEM stubs using double-sided carbon tape and coated with Au/Pd for 30 seconds using an EM ACE200 sputtercoater (Leica, Buffalo Grove, IL, USA). Images were collected with a JSM-6010LA SEM (JEOL, Tokyo, Japan). Analysis of fibers and pores in each gel were quantified using DiameterJ [30 (link)]. Each greyscale SEM image was segmented using the DiameterJ traditional segmentation algorithm to produce eight 8-bit black and white segmentations per SEM image. The best segmentation of the eight was selected based on the following criteria: (1) no partial fiber segmentations, (2) the intersections of fibers do not contain black spots (i.e. holes), (3) segmented fibers are representative of actual fibers in the image and are not background/imaging artifacts, and (4) segmentations accurately represent fibers’ actual diameter. The selected segmentations were scaled and processed in DiameterJ to quantify pore and fiber characteristics.
8
Fractured Sample Microstructure Imaging
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A scanning electron microscope (SEM) (JSM-6010LA SEM; JEOL Ltd., Tokyo, Japan) was used to observe the fractured samples of Modes A and B. A digital microscope Keyence VHX VH-Z20R (Keyence Corporation of America, Itasca, IL, USA) of lower magnification was used to observe the extent of microstructure affected by the treatment modification.
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