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Mouse anti arf antibody

Manufactured by Cell Signaling Technology
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The Mouse anti-ARF antibody is a primary antibody that specifically recognizes the ARF (ADP-Ribosylation Factor) protein. ARF is a small GTPase that plays a crucial role in the regulation of intracellular vesicle trafficking and membrane dynamics. The Mouse anti-ARF antibody can be used to detect and study the expression and localization of the ARF protein in various cell and tissue samples.

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Lab products found in correlation

Rabbit anti ns antibody, by Santa Cruz Biotechnology (2 mentions) Fitc conjutated anti mouse and rhodamine conjugated anti rabbit igg, by Vector Laboratories (2 mentions)

2 protocols using mouse anti arf antibody

1

Characterization of ARF and NS in H1299 Cells

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H1299 cells were transfected with scramble DNA, NS-siRNA or NPM-siRNA. 48 hours after transfection, cells were fixed in 4% PFA for 25 minutes, followed by treatment in 0.3% Triton X-100 for 20 minutes. The fixed cells were blocked with 5% BSA for 30 minutes, the cells were incubated with a mouse anti-ARF antibody (Cell signaling, CA) and a rabbit anti-NS antibody (Santa Cruz) at 4°C overnight. FITC-conjutated anti-mouse and Rhodamine-conjugated anti-rabbit IgG (Vector Laboratories, Burlingame, CA) were used for ARF and NS detection, and DAPI for nuclear staining. The cellular localization of NS/ARF was examined under a fluorescence microscope and double-negative cells were counted. At least six randomly chosen fields of view were examined with a minimum number of 800 cells scored for each condition.
Lo D., Zhang Y., Dai M.S., Sun X.X., Zeng S.X, & Lu H. (2014). Nucleostemin stabilizes ARF by inhibiting the ubiquitin ligase ULF. Oncogene, 34(13), 1688-1697.
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2

Characterization of ARF and NS in H1299 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
H1299 cells were transfected with scramble DNA, NS-siRNA or NPM-siRNA. 48 hours after transfection, cells were fixed in 4% PFA for 25 minutes, followed by treatment in 0.3% Triton X-100 for 20 minutes. The fixed cells were blocked with 5% BSA for 30 minutes, the cells were incubated with a mouse anti-ARF antibody (Cell signaling, CA) and a rabbit anti-NS antibody (Santa Cruz) at 4°C overnight. FITC-conjutated anti-mouse and Rhodamine-conjugated anti-rabbit IgG (Vector Laboratories, Burlingame, CA) were used for ARF and NS detection, and DAPI for nuclear staining. The cellular localization of NS/ARF was examined under a fluorescence microscope and double-negative cells were counted. At least six randomly chosen fields of view were examined with a minimum number of 800 cells scored for each condition.
Lo D., Zhang Y., Dai M.S., Sun X.X., Zeng S.X, & Lu H. (2014). Nucleostemin stabilizes ARF by inhibiting the ubiquitin ligase ULF. Oncogene, 34(13), 1688-1697.
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