Caco-2 cells were purchased from European Collection of Cell Cultures (ECACC, Public Health England Porton Down, Salisbury, UK). Cell medium, chemicals and reagents used for cell culture, and TcdA were purchased from Sigma–Aldrich (St. Louis, MO, USA), unless otherwise stated. Instruments, reagents, and materials used for western blot analysis were obtained from Bio-Rad Laboratories (Milan, Italy). Rabbit anti-zona occludens-1 (ZO-1), anti-occludin and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibodies were procured from Cell Signaling Technology (Danvers, MA, USA). Rabbit anti-toll-like receptor 4 (TLR4), mouse anti-ZO-1, anti-Bcl-2-associated X protein (Bax), mouse anti-MyD88, rabbit anti-transforming growth factor-β-activated kinase-1 (pTAK1), and mouse anti-TAK1 antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and horseradish peroxidase (HRP) was obtained from Dako (Milan, Italy). Fluorescein isothiocyanate-conjugated anti-rabbit antibody and Texas red conjugated anti-mouse antibody were purchased from Abcam (Cambridge, UK), and custom oligonucleotides for electrophoretic mobility shift assay (EMSA) analysis were synthesized by TIB Molbiol (Berlin, Germany).
Anti bcl 2 associated x protein bax
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-Bcl-2-associated X protein (Bax) is a laboratory reagent used in research applications. Bax is a protein that plays a role in apoptosis, or programmed cell death, and is involved in regulating this cellular process. This product provides a source of the Bax protein for use in experimentation and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Anti glyceraldehyde 3 phosphate dehydrogenase gapdh, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase hrp, by Agilent Technologies (1 mentions)
Anti mouse myd88, by Santa Cruz Biotechnology (1 mentions)
Fluorescein isothiocyanate conjugated anti rabbit antibody, by Abcam (1 mentions)
Caco 2 cells, by European Collection of Cell Cultures (1 mentions)
Anti notch1, by Abcam (1 mentions)
Anti hes1, by Abcam (1 mentions)
Anti gapdh, by Thermo Fisher Scientific (1 mentions)
Anti atf6, by Santa Cruz Biotechnology (1 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Anti caspase 12, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase conjugated goat anti rabbit secondary antibody, by Thermo Fisher Scientific (1 mentions)
Anti c ebp homologous protein chop, by Santa Cruz Biotechnology (1 mentions)
Anti pgc 1α, by Abcam (1 mentions)
Anti vascular endothelial cadherin, by Santa Cruz Biotechnology (1 mentions)
Anti b cell lymphoma 2 bcl 2, by Santa Cruz Biotechnology (1 mentions)
Matrigel, by BD (1 mentions)
Anti β actin, by Santa Cruz Biotechnology (1 mentions)
Mouse monoclonal anti jnk, by Santa Cruz Biotechnology (1 mentions)
Bicinchoninic acid bca protein assay kit, by Tiangen Biotech (1 mentions)
4 protocols using anti bcl 2 associated x protein bax
1
Caco-2 Cell Culture and Western Blot Analysis
2
Western Blot Analysis of Kidney Protein Markers
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Equal amounts of protein samples from mouse kidney were separated by SDS-PAGE, transferred onto polyvinylidene fluoride membranes by electroblotting and incubated at 4 °C overnight with primary antibodies (anti-PGC-1α (Abcam, Cambridge, UK), anti-ATF6 (Santa Cruz Biotechnology, USA), anti-C/EBP homologous protein (CHOP, Santa Cruz Biotechnology, USA), anti- phospho-IRE1α (p- IRE1α, Invitrogen, USA), anti-phospho-PERK (p-PERK, Invitrogen, USA), anti-cleaved Poly (ADP-ribose) polymerase 1 (c-PARP1, Santa Cruz Biotechnology, USA), anti-mitofusin 2 (Mfn2, Abcam, Cambridge, UK), anti-apoptotic protein B-cell lymphoma 2 (Bcl2, Santa Cruz Biotechnology, USA), anti-Bcl2-associated X protein (Bax, Santa Cruz Biotechnology, USA), anti-cleaved caspase 3 (Cell Signaling Technology, USA), anti-caspase 12 (Cell Signaling Technology, USA), anti-Notch1 (Abcam, Cambridge, UK), anti-Hes1(Abcam, Cambridge, UK) and anti-GAPDH (Invitrogen, USA)). Next, the membranes were incubated with horseradish peroxidase–conjugated goat anti–rabbit secondary antibody (Invitrogen, USA) at 25 °C for 1.5 h. The chemiluminescent bands were visualized using a chemiluminescence detection system and quantified using ImageJ software.
3
Angiogenesis Inhibition by Arsenic Trioxide
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The As2O3 solution was purchased from Heilongjiang Harbin Medical Pharmaceutical Co., Ltd. (Heilongjiang, China). Matrigel was purchased from BD Biosciences (Franklin Lakes, NJ, USA), the CD105-antibody was purchased from Thermo Fisher Scientific, Inc. (Waltham, MA, USA), and anti-vascular endothelial (VE)-cadherin, anti-Bcl-2-associated X protein (Bax) and anti-B-cell lymphoma 2 (Bcl-2) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Matrix metalloproteinase (MMP)-2 and MMP-9 antibodies were purchased from Nanjing KeyGen Biotech Co., Ltd. (Nanjing, China), and the animals were obtained from Hunan Slack Jingda Experimental Animals Co., Ltd. (Hunan, China).
4
Ginsenoside Rg1 Mitigates Diabetic Pathology
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The study protocol was approved by the Animal Care and Use Committee of Kunming Medical University. Male 4-week-old Sprague Dawley rats (N = 80, 200 ± 20 g) were purchased from the Animal Center of Kunming Medical University. The rats were housed at 23° ± 2°C and 55 ± 2% humidity. The animals had free access to tap water and normal rat diet prior to the start of the experiment. Ginsenoside Rg1 of >98% purity measured via high-performance liquid chromatography was obtained from the College of Pharmacy of Kunming Medical University. STZ was obtained from Sigma Chemical Co. (St. Louis, MO, USA). The bicinchoninic acid (BCA) protein assay kit was obtained from TIANGEN Biotech (Beijing) Co., Ltd. Glucose and insulin enzyme-linked immunosorbent assay (ELISA) kits were purchased from Bio-Swamp Life Science Lab, China. Mouse monoclonal anti-JNK, anti-phospho-JNK (p-JNK), anti-caspase-3, anti-BCL-2-associated X protein (BAX), anti-B cell leukemia/lymphoma 2 (BCL-2), and anti-b-actin were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Goat anti-mouse antibody labeled with horseradish peroxidase was obtained from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China.
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