The antibodies used in this study were as follows: USP28 (17707-1-AP, 1:1000 WB, Proteintech); RECQL1 (A300-450A, 1:1000 WB, Bethyl Lab, Montgomery); WRN (4666S,1:1000 WB,Cell Signaling); BLM (A300-110A,1:1000 WB, Bethyl Lab); RECQL4 (17008-1-AP,1:1000 WB, Proteintech); RECQL5 (A302-520A, 1:2000 WB, Bethyl Lab); Flag (F3165, 1:2000, Sigma); Ub (SC-8017, 1:200 WB, Santa Cruz Biotechnology); γH2AX (05-636, 1:500 IF, Millipore); Claspin (2800S, 1:1000 WB, Cell Signaling); c-MYC (SC-40, 1:200 WB, Santa Cruz); Phospho-Chk1-Ser345 (2348, 1:1000 WB, Cell Signaling); CHK1 (ab32531, 1:1000 WB, Abcam); GAPDH (60004-1-1g, 1:5000 WB, Proteintech); Tubulin (66240-1-1g, 1:5000 WB, Proteintech); ACTIN (66009-1-1g, 1:3000 WB, Proteintech). The secondary antibodies conjugated to horseradish peroxidase for Western blot and the secondary antibodies anti-mouse, -goat or -rabbit Alexa fluor 488 or 594 for immunofluorescence staining were purchased from Jackson ImmunoResearch Laboratories. siRNAs were synthesized by GenePharma.
Ub sc 8017
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The Ub (sc-8017) is an antibody product offered by Santa Cruz Biotechnology. It is a primary antibody that specifically recognizes ubiquitin, a highly conserved protein involved in various cellular processes. The antibody can be used for the detection and analysis of ubiquitin in various applications, such as Western blotting, immunoprecipitation, and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Phospho chk1 ser345 2348, by Cell Signaling Technology (1 mentions)
Flag f3165, by Merck Group (1 mentions)
γh2ax 05 636, by Merck Group (1 mentions)
C myc sc 40, by Santa Cruz Biotechnology (1 mentions)
Usp28 17707 1 ap, by Proteintech (1 mentions)
Recql1 a300 450a, by Fortis Life Sciences (1 mentions)
Wrn 4666s, by Cell Signaling Technology (1 mentions)
Blm a300 110a, by Fortis Life Sciences (1 mentions)
Gapdh 60004 1 1g, by Proteintech (1 mentions)
Nanog ab109250, by Abcam (1 mentions)
Gapdh 2118l, by Cell Signaling Technology (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Enhanced chemiluminescence reagent, by Thermo Fisher Scientific (1 mentions)
Hmgb1, by Merck Group (1 mentions)
Flag tag (flag), by Proteintech (1 mentions)
β tubulin, by Proteintech (1 mentions)
Ha tag, by Proteintech (1 mentions)
Anti β actin 20536 1 ap, by Proteintech (1 mentions)
4 protocols using ub sc 8017
1
Antibody Characterization for Helicase Enzymes
2
Multiparametric Antibody Detection Protocol
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SOX2 (2748), MEK1 (12671), phospho-MEK1 (2338), Ki-67 (8D5) (9449) and GAPDH (2118L) antibodies were purchased from Cell Signaling Technology. SIRT1 (H-300) (sc-15404), c-Myc (9E10) (sc-40) and Ub (sc-8017) antibodies were purchase from Santa Cruz. GFP (ab290), OCT4 (ab19857) and Nanog (ab109250) antibodies were purchased from abcam. ERK1/2 (05-1152) and phospho- ERK1/2 (05-797R) were purchased from Millpore. Alexa Flour 647 (A31571) antibody was purchased from life technologies.
3
Western Blot Analysis of Protein Modifications
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Whole-cell lysates (WCL) were collected using the protein lysis buffer containing proteinase and phosphatase inhibitors. The BCA assay was operated to measure the protein concentration. The protein was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels and transferred to polyvinylidene difluoride (PVDF) membranes (0.25 μm, Millipore). The membranes were then blotted with primary antibodies followed by the secondary antibody and developed with enhanced chemiluminescence reagent (Invitrogen). The primary antibodies used in this study included USP15 (67557-1-Ig, Proteintech), HMGB1 (SAB1403925, Sigma), Ub (sc-8017, Santa Cruz), HA-tag (51064-2-AP, Proteintech), Flag-tag (66008-3-Ig, Proteintech), His-tag (12698, CST), and β-tubulin (MA5-16308, Proteintech). The relative protein quantification was analyzed using ImageJ software.
4
Comprehensive Immunoblotting Assay Protocol
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Antibodies against SRA (sc-166184) and Ub (sc-8017) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-HA (T0050) was from Affinity Biosciences (Changzhou, Jiangsu, China). The anti-Flag antibody (HT201) was obtained from TransGen Bio-tech (Beijing, China). The anti-V5 (66007-1-Ig), anti-Myc (66004-1-Ig), and anti-β-actin (20536-1-AP) were purchased from Proteintech (Rosemont, IL, USA). Antibodies obtained from Cell Signaling Technology (Danvers, MA, USA) included the antibodies against phosphor-TBK1(5483), TBK1(38066), phospho-IRF3(29047), and IRF3(4302). K63-linkage-specific polyubiquitin (5621) was purchased from Sigma-Aldrich. A human IFN-β enzyme-linked immunosorbent assay (ELISA) kit (JL19215) was purchased from Jianglai Biotechnology (Shanghai, China). Percoll (P1644) was purchased from Sigma-Aldrich (St. Louis, MO, USA). USP15 siRNA was synthesized from RiboBio (Guangzhou, China).
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