The antibodies used in this study and their sources are as follows: rabbit anti-MAP2 (Santa Cruz Biotechnology, Dallas, TX); goat anti–ankyrin G (Santa Cruz Biotechnology); chicken anti-GFP (Invitrogen, Carlsbad, CA); mouse anti-TGN38 (Thermo Scientific, Rockford, IL); mouse anti–γ-adaptin (BD Biosciences, San Diego, CA); mouse anti-HA (Covance, Dedham, MA); chicken anti-HA (Millipore, Billerica, MA); chicken anti-MAP2 (Abcam, Cambridge, MA); and mouse anti–pan-neurofascin (external epitope, Clone A12/18; University of California, Davis/National Institutes of Health NeuroMab Facility, Bethesda, MD). Rabbit anti-HA and rabbit anti-myc were gifts from A. Sharma (National Institutes of Health). The antibody to p230 was a gift from M. Krieger (MIT, Cambridge, MA). Rabbit anti-GST antiserum and mouse anti-myc clone 9E10 have been described before (Dell’Angelica et al., 1998 (link); Mattera et al., 2011 (link)). Mix-n-stain-CF640R (Biotium, Hayward, CA) was used to label the antibody to neurofascin.
Goat anti ankyrin g
Manufactured by Santa Cruz Biotechnology
Goat anti–ankyrin G is a primary antibody that specifically recognizes ankyrin G, a protein involved in the organization and function of the cytoskeleton. This antibody is generated in goats and can be used to detect and study the expression and localization of ankyrin G in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Chicken anti gfp, by Thermo Fisher Scientific (1 mentions)
Mouse anti γ adaptin, by BD (1 mentions)
Chicken anti ha, by Merck Group (1 mentions)
Mix n stain cf640r, by Biotium (1 mentions)
Rabbit anti map2, by Santa Cruz Biotechnology (1 mentions)
Chicken anti map2, by Abcam (1 mentions)
Mouse anti ha, by Fortrea (1 mentions)
Rabbit anti calretinin, by Swant (1 mentions)
Pegfp n1 vector, by Takara Bio (1 mentions)
Mouse anti gm130, by BD (1 mentions)
2 protocols using goat anti ankyrin g
1
Antibody Sources for Neuroscience Research
2
Molecular Tools for Neuroscience Research
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pCA-EGFP and pAAV-CAG-TdTomato were previously described [20 (link), 21 (link)]. pAcGFP1-Golgi was obtained from Clontech. For construction of pCAGGS-GRASP65-GFP, cDNA encoding GRASP65 was cloned by PCR from a cDNA library. The PCR product was subcloned into pEGFP-N1 vector (Clontech), and the EGFP-fused GRASP65 was subcloned into pCAGGS vector. Antibodies used for immunostaining were as follows: rabbit anti-Math2, anti-Prox1 and anti-Synapsin I, chick anti-NeuN (Abcam); mouse anti-GM130 (BD Biosciences); rabbit anti-Calbindin, anti-GFAP and mouse anti-GAD67 (Chemicon); mouse anti-PSD95 (Funakoshi); mouse anti-Prox1 (Millipore); goat anti-Ankyrin G (Santa Cruz); rabbit anti-Calretinin (Swant); Alexa405-, Alexa488-, Alexa568-, Alexa633- or Alexa647-conjugated anti-chick, anti-goat, anti-mouse or anti-rabbit IgG (Molecular Probes).
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