Lsm 510 multiphoton microscope

Manufactured by Zeiss

The LSM 510 multiphoton microscope is a high-performance imaging system designed for advanced biological research. It utilizes multiphoton excitation technology to enable deep tissue imaging with reduced phototoxicity and photobleaching. The microscope provides high-resolution, three-dimensional visualization of live samples.

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Lab products found in correlation

Diaminophenylindole dapi, by Merck Group (1 mentions) Dmi6000 microscope, by Molecular Devices (1 mentions) Bz x810 microscope, by Keyence (1 mentions) Bz x analyzer software, by Keyence (1 mentions) Metamorph software, by Molecular Devices (1 mentions) Indo 1am dye, by Thermo Fisher Scientific (1 mentions) Volocity software, by PerkinElmer (1 mentions)

Close spelling variants of this product

LSM 510 multiphoton confocal microscope LSM 510 Meta Multiphoton Confocal Microscope Zeiss LSM 510 Meta Multiphoton microscope LSM 510 Multiphoton Laser Scanning Microscope LSM 510 Meta NLO multiphoton confocal microscope LSM 510 NLO multiphoton microscope LSM 510 microscope LSM 510

5 protocols using lsm 510 multiphoton microscope

Brightfield, Phase Contrast, and Confocal Immunofluorescence Imaging Protocols

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Brightfield images were obtained with oblique illumination and a 10X PlanFluor_DL 0.30/15.20mm Ph1 objective using a Keyence BZ-X810 microscope and BZ-X Analyzer software. 25 fields were captured from biological triplicates for each treatment, with one representative image per sample presented in figures. Phase contrast images were captured at 10X magnification using a Leica DMI6000 microscope and MetaMorph software (Molecular Devices). Confocal immunofluorescence analysis was conducted as previously described^{16 (link), 19 (link)} using 1 μg/mL Diaminophenylindole (DAPI) (Sigma-Aldrich, cat. # D9542) to stain nuclei and the Alexafluor-conjugated primary antibodies and dilutions indicated in Supplementary Table 1. Confocal microscopy mages were captured using AIM software (Leica) with a Zeiss LSM 510 multiphoton microscope and 63X oil immersion objective.

Bryson B.L., Tamagno I., Taylor S.E., Parameswaran N., Chernosky N.M., Balasubramaniam N, & Jackson M.W. (2020). Aberrant induction of a mesenchymal/stem-cell program engages senescence in normal mammary epithelial cells.. Molecular cancer research : MCR, 19(4), 651-666.

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Assessing Neuronal Calcium Dynamics with Amyloid-beta Oligomers

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At 12–14 days in vitro (DIV) or 21 DIV, neuronal cultures were incubated with 6 µM indo-1/AM dye (Invitrogen) and 2 µM sulforhodamine 101 (SR101; Life Technologies) for 45 min at 37 °C. Images were acquired using a 25× water immersion objective on an inverted Zeiss LSM 510 multiphoton microscope with a humidified environmental chamber that maintained temperature at 37 °C. Two-photon excitation was performed at 750 nm using a mode-locked Chameleon Ti:sapphire laser (Coherent, Inc) as the source. Ratiometric images were generated with the simultaneous acquisition using non-descanned detectors of two spectral channels: 390–465 nm and 480–522 nm. Multiple fields were acquired that included 300–400 cells per dish. After baseline calcium was obtained, the cultures were treated with antibody-immunodepleted AβOs or AβOs-alone by replacing half of the total volume of the media in the dish with oligomer-containing media for 45 min. The cultures were then re-imaged in the same areas in the dish.

Wang X., Kastanenka K.V., Arbel-Ornath M., Commins C., Kuzuya A., Lariviere A.J., Krafft G.A., Hefti F., Jerecic J, & Bacskai B.J. (2018). An acute functional screen identifies an effective antibody targeting amyloid-β oligomers based on calcium imaging. Scientific Reports, 8, 4634.

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Retinal Cell Identification in Mice

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All animal procedures were conducted in accordance with NIH guidelines, as approved by the NINDS Animal Care and Use Committee. Retinas were isolated from eGFP-DRD4/Chat-Cre/TdTomato (RRID:MMRRC_000231-UNC) mice (postnatal days 14 70). All subsequent procedures and recordings were performed in Ames media or artificial cerebrospinal fluid equilibrated with 95% O₂/5% CO₂. Recordings were performed in ambient light levels to reduce rod-pathway activation. All experiments were performed at ~35 °C. Cells were identified by their GFP expression using a Zeiss LSM-510 multiphoton microscope.

Poleg-Polsky A, & Diamond J.S. (2016). NMDA receptors multiplicatively scale visual signals and enhance directional motion discrimination in retinal ganglion cells. Neuron, 89(6), 1277-1290.

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Far-Red Light Microscopy of Seedlings

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For fluorescence microscopy analyses, seedlings were grown on MS medium for 5 d and then released to far-red light for 10 h. At least 10 independent lines for each cross combination were examined using a Zeiss LSM 510 multiphoton microscope.

Liu Y., Sun Y., Yao H., Zheng Y., Cao S, & Wang H. (2022). Arabidopsis Circadian Clock Repress Phytochrome a Signaling. Frontiers in Plant Science, 13, 809563.

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Multiphoton Imaging of Fresh Liver Tissue

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Freshly removed liver tissue was placed in 35mm coverslip-bottom Petri dishes (MatTek corporation), kept moist with ice cold RPMI 1640 and imaged on an inverted LSM 510 multiphoton microscope (Carl Zeiss Microimaging). Images were acquired with a 40x 1.1 water immersion objective and fluorescence excitation provided by a Chameleon XR Ti:sapphire laser (Coherent) tuned to ~870-900nm. For 3D analysis, 20–40μm Z stacks were acquired with a Z distance of 2–3μm. Images were rendered and analysed using Volocity software (Improvision).

Moore J.W., Beattie L., Osman M., Owens B.M., Brown N., Dalton J.E., Maroof A, & Kaye P.M. (2016). CD4+ Recent Thymic Emigrants Are Recruited into Granulomas during Leishmania donovani Infection but Have Limited Capacity for Cytokine Production. PLoS ONE, 11(9), e0163604.

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