Rabbit anti human ccbe1

For immunofluorescence studies, specimens were fixed for 20–25 min in 4% paraformaldehyde (PFA), rinsed in PBS, transferred into 10% and 30% sucrose in PBS, and embedded in tissue freeze medium (Tissue Tek, Sakura Finetek Zoeterwoude, NL). Sections of 12–14μm were incubated with the following primary antibodies: Rabbit-anti-human CCBE1 (1:500, Sigma-Aldrich, München, Germany), rabbit-anti-human ESAM-1 (1:100, Sigma-Aldrich, München, Germany), goat-anti-human smooth muscle α-actin (SMA) (Acris Antibodies, Herford, Germany), mouse-anti-human CD31 (1:50, BD Pharmingen), mouse-anti-human CD117 (c-KIT, 1:100, Santa Cruz Biotechnol., and 1:1, Dako, Hamburg, Germany), mouse-anti-human D2-40 (1:200, Dako, Hamburg, Germany), rabbit-anti-human Lyve-1 (1:500, ReliaTech, Braunschweig, Germany), rabbit-anti-human Prox1 (1:500, ReliaTech, Braunschweig, Germany), mouse-anti-human vimentin (1:200, Dako), mouse-anti-human PDGFRα (1:1, Dako), mouse-anti-human CD34 (1:200, Dako). Secondary antibodies were: goat-anti-mouse Alexa 488/594, goat-anti-rabbit Alexa 594, donkey-anti-goat Alexa 488 (MobiTech, Göttingen, Germany). Sections were counter-stained with Dapi and mounted under cover slips with Fluoromount-G (Southern Biotechnology, US). Photos were taken with AxioImagerZ1 (Zeiss, Göttingen, Germany).

For immunofluorescence studies, specimens were fixed for 20–25 min in 4% paraformaldehyde (PFA), rinsed in PBS, transferred into 10 and 30% sucrose in PBS, and embedded in tissue freeze medium (Tissue Tek, Sakura Finetek Zoeterwoude, NL). Sections of 12 – 14 μm were incubated with the following primary antibodies: Rabbit-anti-human CCBE1 (1:500, Sigma-Aldrich, München, Germany; #R38605), mouse-anti-human CD31 (1:50, BD Pharmingen; clone WM59), mouse-anti-human D2–40/podoplanin (1:200, Dako, Hamburg, Germany; #M3619), rabbit-anti-human LYVE-1 (1:500, ReliaTech, Wolfenbüttel, Germany; #102-PA50AG), rabbit-anti-human PROX1 (1:500, ReliaTech; #102-PA32AG), mouse-anti-human vimentin (1:200, Dako; #GA630), mouse-anti-human CD34 (1:200, Dako; clone QBEnd-10), mouse-anti-human VEGFR-3 (1:100, ReliaTech; #101-M36). Secondary antibodies were: goat-anti-mouse Alexa 488/594 (#A11001; #A21135), goat-anti-rabbit Alexa 594 (#A11012), donkey-anti-goat Alexa 488 (#A11055; MobiTech, Göttingen, Germany). Sections were counter-stained with Dapi and mounted under cover slips with Fluoromount-G (Southern Biotechnology, US). Photos were taken with AxioImagerZ1 (Zeiss, Göttingen, Germany).