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Mouse methocult medium

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Mouse MethoCult medium is a semisolid culture medium used for the colony-forming unit (CFU) assay to quantify the number of hematopoietic progenitor cells in mouse bone marrow, spleen, and other tissue samples.

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Gelcount analyzer, by Oxford Optronix (2 mentions)

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MethoCult (224 citations) MethoCult medium (57 citations)

5 protocols using mouse methocult medium

1

BCR-ABL1-transformed Mouse Pre-B ALL Cell Colony Assay

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The methylcellulose colony-forming assays were performed with 10,000 BCR-ABL1-transformed mouse pre-B ALL cells. Cells were resuspended in mouse MethoCult medium (StemCell Technologies) and cultured on 3-cm diameter dishes, with an extra water supply dish to prevent evaporation. After 21 days, colony numbers were counted by a GelCount analyzer (Oxford Optronix).
Buchner M., Park E., Geng H., Klemm L., Flach J., Passegué E., Schjerven H., Melnick A., Paietta E., Kopanja D., Raychaudhuri P, & Müschen M. (2015). Identification of FOXM1 as a therapeutic target in B-cell lineage acute lymphoblastic leukaemia. Nature Communications, 6, 6471.
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2

Methylcellulose Assay for Mouse HSPCs

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We analyzed methylcellulose colony formation using 100,000 mouse HSPCs. Specifically, the mouse MethoCult medium (StemCell Technologies) was used for preparing the cell suspension. Then, cell culture was performed using culture plates (3 cm in diameter), and evaporation was prevented through the addition of excessive PBS. The number of colonies formed was counted after 14 days.
Yin Z., Ou R., Zhu Y., Liu Z., Huang J., Zhong Q., Li G., Zhang Q, & Liu S. (2024). Coniferyl ferulate alleviate xylene-caused hematopoietic stem and progenitor cell toxicity by Mgst2. Frontiers in Pharmacology, 15, 1334445.
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3

Evaluating BCR-ABL1 Pre-B ALL Cells

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The methylcellulose colony-forming assays were performed with 10,000 BCR-ABL1 pre-B ALL cells. Cells were resuspended in mouse MethoCult medium (StemCell Technologies) and cultured on 3-cm diameter dishes, with an extra water supply dish to prevent evaporation. Images were taken and colony numbers were counted after 14 days.
Chan L.N., Chen Z., Braas D., Lee J.W., Xiao G., Geng H., Cosgun K.N., Hurtz C., Shojaee S., Cazzaniga V., Schjerven H., Ernst T., Hochhaus A., Kornblau S.M., Konopleva M., Pufall M.A., Cazzaniga G., Liu G.J., Milne T.A., Koeffler H.P., Ross T.S., Sanchez-Garcia I., Borkhardt A., Yamamoto K.R., Dickins R.A., Graeber T.G, & Müschen M. (2017). Metabolic gatekeeper function of B-lymphoid transcription factors. Nature, 542(7642), 479-483.
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4

BCR-ABL1-transformed Mouse Pre-B ALL Cell Colony Assay

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The methylcellulose colony-forming assays were performed with 10,000 BCR-ABL1-transformed mouse pre-B ALL cells. Cells were resuspended in mouse MethoCult medium (StemCell Technologies) and cultured on 3-cm diameter dishes, with an extra water supply dish to prevent evaporation. After 21 days, colony numbers were counted by a GelCount analyzer (Oxford Optronix).
Buchner M., Park E., Geng H., Klemm L., Flach J., Passegué E., Schjerven H., Melnick A., Paietta E., Kopanja D., Raychaudhuri P, & Müschen M. (2015). Identification of FOXM1 as a therapeutic target in B-cell lineage acute lymphoblastic leukaemia. Nature Communications, 6, 6471.
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5

Evaluating BCR-ABL1 Pre-B ALL Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The methylcellulose colony-forming assays were performed with 10,000 BCR-ABL1 pre-B ALL cells. Cells were resuspended in mouse MethoCult medium (StemCell Technologies) and cultured on 3-cm diameter dishes, with an extra water supply dish to prevent evaporation. Images were taken and colony numbers were counted after 14 days.
Chan L.N., Chen Z., Braas D., Lee J.W., Xiao G., Geng H., Cosgun K.N., Hurtz C., Shojaee S., Cazzaniga V., Schjerven H., Ernst T., Hochhaus A., Kornblau S.M., Konopleva M., Pufall M.A., Cazzaniga G., Liu G.J., Milne T.A., Koeffler H.P., Ross T.S., Sanchez-Garcia I., Borkhardt A., Yamamoto K.R., Dickins R.A., Graeber T.G, & Müschen M. (2017). Metabolic gatekeeper function of B-lymphoid transcription factors. Nature, 542(7642), 479-483.
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