The standard laboratory stocks yw, w1118, and Canton-S were originally obtained from the Bloomington Stock Center. The OK72-Gal4 line, UAS-kir2.1 line, UAS-Gal80ts line, and Sir2 mutant lines were also obtained from the Bloomington Stock Center. UAS-TRA and UAS-TRADSRNA were provided by B.J. Dickson. The ppk23 mutant line was provided by K. Scott. The npf mutant line was created as described below. The Crz-Gal4 line was provided by E. Johnson. Thus UAS-TrpA1 line was provided by P. Garrity. The dFoxo and dILP mutant lines were provided by L. Partridge. The tagged-dILP2 line was provided by S. Kim. The AkhR mutants and revertant controls were provided by R. Kühnlein. All lines except those used solely in the downstream effector screen (Table S2 ) were backcrossed at least 8 generations. dFoxo mutants were backcrossed following the effector screen, and all data not appearing within the table (i.e., in Figure 4 and Figure S7 ) were obtained using backcrossed lines.
Uas gal80ts line
Manufactured by Bloomington Drosophila Stock Center
The UAS-Gal80ts line is a genetic tool used in Drosophila research. It allows for temporal and spatial control of gene expression. The Gal80ts protein, a temperature-sensitive repressor of the Gal4 transcription factor, regulates the activity of UAS-driven transgenes.
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2 protocols using uas gal80ts line
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Drosophila Genetic Stocks Provenance
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Drosophila Genetic Stocks Provenance
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The standard laboratory stocks yw, w1118, and Canton-S were originally obtained from the Bloomington Stock Center. The OK72-Gal4 line, UAS-kir2.1 line, UAS-Gal80ts line, and Sir2 mutant lines were also obtained from the Bloomington Stock Center. UAS-TRA and UAS-TRADSRNA were provided by B.J. Dickson. The ppk23 mutant line was provided by K. Scott. The npf mutant line was created as described below. The Crz-Gal4 line was provided by E. Johnson. Thus UAS-TrpA1 line was provided by P. Garrity. The dFoxo and dILP mutant lines were provided by L. Partridge. The tagged-dILP2 line was provided by S. Kim. The AkhR mutants and revertant controls were provided by R. Kühnlein. All lines except those used solely in the downstream effector screen (Table S2 ) were backcrossed at least 8 generations. dFoxo mutants were backcrossed following the effector screen, and all data not appearing within the table (i.e., in Figure 4 and Figure S7 ) were obtained using backcrossed lines.
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