Human igg fc antibody

Manufactured by Fortis Life Sciences

The human IgG-Fc antibody is a laboratory product that represents the Fc region of the human immunoglobulin G (IgG) antibody. The Fc region is responsible for the effector functions of the antibody, such as complement activation and Fc receptor binding. This product can be used for various research and analytical applications involving the study of the Fc region and its interactions.

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Lab products found in correlation

Fibrinogen, by Merck Group (1 mentions) Soluble fibrin, by Sekisui (1 mentions) Anti mouse igg fc antibody, by Fortis Life Sciences (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Sodium chloride (nacl), by Avantor (1 mentions) Sodium hydroxide (naoh), by Avantor (1 mentions) Tetramethylbenzidine tmb substrate, by Thermo Fisher Scientific (1 mentions) Celltiter 96 mtt, by Promega (1 mentions) Tris hydrochloride, by Avantor (1 mentions) Rpmi 1640 medium, by Merck Group (1 mentions) Formic acid, by Merck Group (1 mentions) Dibasic sodium phosphate heptahydrate, by Avantor (1 mentions) Monobasic sodium phosphate monohydrate, by Avantor (1 mentions) Pngase f, by New England Biolabs (1 mentions) Mabthera, by Roche (1 mentions) Adcc reporter bioassay kit, by Promega (1 mentions)

Spelling variants of this product

HRP-conjugated goat anti-human IgG-Fc antibody (4 citations) Goat anti-human IgG-Fc fragment antibody (2 citations) Goat anti-human IgG-Fc fragment cross-adsorbed antibody (2 citations)

2 protocols using human igg fc antibody

Fibrinogen-Binding Antibody Assay

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Fibrinogen (Sigma-Aldrich), soluble fibrin (Sekisui Medical), and FDP (Sekisui Medical), were also immobilized on 96-well plates (1 μg/well of each compound). The plate was reacted with antibody solution (1 μg/mL) for 1 hour, washed with TBS-T, reacted with horseradish peroxidase (HRP)-conjugated anti-human IgG-Fc antibody or anti-mouse IgG-Fc antibody (Bethyl) for 1 hour, and washed with TBS-T. After incubation with the TMB (3,3′,5,5′-tetramethylbenzidine) color-developing reagent for 15 minutes, the reaction was stopped by addition of 2 N H
₂SO
₄, and absorbance at 450 nm was measured. The antibodies used in this experiment were 1101, the negative control, and the previously established 59D8 mAb, which recognizes the N-terminus of Fibrinogen after thrombin cleavage.

Hanaoka S., Saijou S, & Matsumura Y. (2021). A Novel and Potent Thrombolytic Fusion Protein Consisting of Anti-Insoluble Fibrin Antibody and Mutated Urokinase. Thrombosis and Haemostasis, 122(1), 57-66.

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Rituximab Biosimilar Comparative Analysis

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Dibasic sodium phosphate heptahydrate (Na₂HPO₄·7H₂O), monobasic sodium phosphate monohydrate (NaH₂PO₄·H₂O), sodium chloride (NaCl), Tris-hydrochloride (NH₂C(C₂OH)₃·HCl), and sodium hydroxide (NaOH) were obtained from J. T. Baker (Center Valley, PA). Sodium azide (NaN₃), ammonium formate (CH₅NO₂), RPMI-1640 medium, fetal bovine serum (FBS), and formic acid were acquired from Sigma-Aldrich (St. Louis, MO). 2-Aminobenzamide (2-AB) was obtained from ProZyme Inc. (Hayward, CA); PNGase F was purchased from New England Biolabs (Woburn, MA) and Human IgG-Fc antibody from Bethyl Laboratories Inc. (Montgomery, TX). Tetramethylbenzidine (TMB) substrate was obtained from Thermo Scientific (Waltham, MA). ADCC Reporter Bioassay Kit and CellTiter 96 MTT were purchased from Promega (Madison, WI). Water was obtained from a Millipore Milli-Q Biocel system (Billerica, MA). All solutions were filtered through 0.2 μm prior to analysis. Two products containing rituximab were employed: Kikuzubam from Probiomed S.A. de C.V., Mexico, and MabThera from F. Hoffmann-La Roche Ltd. Basel, Switzerland, as the reference product.

Miranda-Hernández M.P., López-Morales C.A., Ramírez-Ibáñez N.D., Piña-Lara N., Pérez N.O., Molina-Pérez A., Revilla-Beltri J., Flores-Ortiz L.F, & Medina-Rivero E. (2015). Assessment of Physicochemical Properties of Rituximab Related to Its Immunomodulatory Activity. Journal of Immunology Research, 2015, 910763.

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