Rtca cardio software version 1

An impedance assay was performed using xCELLigence RTCA Cardio system (ACEA Biosciences). This system registers electrical impedance changes across synchronously beating cardiac monolayers and displays these data by converting impedance values into Cell Index (CI) values. The CI value (arbitrary unit) is directly proportional to the surface area covered by the attached cells in the E-plate Cardio 96. This system monitors change in cell morphology, cell viability, beating rate, and beating pattern in real time. Before cell seeding, background impedance readings were measured after the addition of 50 µl of iCell-PM to each well of an E-plate Cardio 96. The hiPSC-CMs plated on the E-plate Cardio 96 underwent a 2-day exposure to five test compounds at various concentrations. Impedance signals from each well of the E-plate Cardio 96 were collected at regular intervals. Cytotoxicity and beating data were analyzed using the RTCA Cardio software version 1.0 (ACEA Biosciences). To evaluate the effects of the five cosmetic test compounds on cell viability and cardiac beating function under a serum-free culture conditions, hiPSC-CMs underwent a 2-day exposure to these five test compounds in serum-free basal culture medium; ‘Dulbeccos’s Modified Eagle Medium’ (DMEM, Invitrogen, Darmstadt, Germany). In control cultures, dimethyl sulfoxide (DMSO) was used as the compound-free solvent.

The xCELLigence RTCA Cardio system (ACEA Biosciences, San Diego, CA, USA) is an impedance-based platform for monitoring the real-time beating function of cardiomyocytes. It was used to sensitively and quantitatively detect pro-arrhythmic drug effects on cardiac function and to measure cell viability in real time. Impedance measurements were monitored at regular time intervals. The amount of growth area covered in an E-plate Cardio 96 due to cell adhesion was represented as the Cell Index (CI). A high CI indicates more cell adhesion. Before cell plating, the background impedance of E-plate Cardio 96 (ACEA Biosciences, San Diego, CA, USA) was measured using iCell-PM (50 µl per well). The raw data of cell viability, beating activity and the beating amplitude were acquired using the xCELLigence RTCA Cardio system and analysed using RTCA Cardio software version 1.0 (ACEA Biosciences, Inc, San Diego, CA, USA).