N methyl n trimethylsilyl trifluroacetamide

A549 human pulmonary epithelial cell line was purchased from the American Type Culture Collection (Manassas, VA, USA). Enoxaparin was obtained from Aventis Pharma Ltd. (NSW, Australia). UFH was purchased from Hospira Pty. Ltd. (Victoria, Australia). Sodium chloride, tetrahydrofuran, sodium hydroxide, N-methyl-N-(trimethylsilyl) trifluroacetamide, methanol, acetic acid, Ham’s F12K medium, antibiotics (penicillin G and streptomycin), trypsin-ethylenediaminetetraacetic acid (EDTA), trypsin, thrombin, enzyme-linked immunosorbent assay (ELISA) kits for IL-6 and IL-8, trypan blue exclusion assay kit, lactate dehydrogenase (LDH) activity assay kit, and potassium phosphate monobasic and dibasic were purchased from Sigma-Aldrich (Castle Hill, NSW, Australia). Fetal bovine serum, IL-6 and IL-8 recombinant human proteins were obtained from Invitrogen (Grand Island, NY, USA). Deuterium oxide (D₂O) was purchased from Cambridge Isotope Laboratories (Andover, MA, USA).

The labeling substrates, [1-¹³C]glucose (99 atom%), [1,2-¹³C]glucose (99 atom%), and [¹³C₆]glucose (99 atom%), were purchased from Sigma-Aldrich. Derivatization agents TBDMS [N-methyl-N-(t-butyldimethylsilyl) trifluoroacetamide plus 1% t-butyl-dimethylchlorosilane] and MSTFA [N-methyl-N-(trimethylsilyl) trifluroacetamide] were also purchased from Sigma-Aldrich. Medium components and other reagents were purchased from Himedia and Sigma-Aldrich.