Anti α1 antitrypsin

Manufactured by Agilent Technologies

Sourced in Denmark, United States

Anti–α1-antitrypsin is a laboratory product used for the detection and measurement of α1-antitrypsin, a protein involved in the regulation of protease activity. It is a key tool for the diagnosis and monitoring of various conditions, including liver and lung disorders.

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Lab products found in correlation

Thapsigargin, by MP Biomedicals (1 mentions) Anti flag m1, by Merck Group (1 mentions) Irdye 680 and 800 nm, by LI COR (1 mentions) Anti bip, by BD (1 mentions) Anti tubulin, by Merck Group (1 mentions) Dithiothreitol (dtt), by Merck Group (1 mentions) Bafilomycin a1, by Merck Group (1 mentions) Anti ha, by Fortrea (1 mentions) Puromycin, by InvivoGen (1 mentions) Anti mouse igg hrp linked antibody, by Jackson ImmunoResearch (1 mentions) Anti firefly luciferase, by Abcam (1 mentions) Anti β actin, by Merck Group (1 mentions) Anti flag m2, by Merck Group (1 mentions) Affinipure goat anti mouse igg h l, by Jackson ImmunoResearch (1 mentions) Anti calnexin, by Stressgen (1 mentions)

2 protocols using anti α1 antitrypsin

ER Stress Protein Regulation Analysis

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The following chemicals were used: thapsigargin (MP Biomedicals, Santa Ana, CA), tunicamycin and
kifunensine (Calbiochem, Billerica, MA), puromycin (InvivoGen, San Diego, CA), and bafilomycin A1,
MG-132, and DTT (Sigma-Aldrich, St. Louis, MO). For Western blot analyses, the following antibodies
were used: anti-FLAG M1 and anti-tubulin (Sigma-Aldrich), anti–α1-antitrypsin (Dako,
Glostrup, Denmark), anti–14-3-3z and anti-SEL1L (Santa Cruz Biotechnology, Dallas, TX),
anti–S-tag and anti–OS-9 (Abcam, Cambridge, England), anti–GRP94 9G10 and
anti-KDEL (Enzo, Farmingdale, NY), anti-GRP94 (provided by Ineke Braakman, Utrecht University,
Utrecht, Netherlands), anti-HA (Covance, Princeton, NJ), anti-BiP (BD Biosciences, San Jose, CA),
and anti-PERK and anti-JNK (Cell Signaling Technologies, Danvers, MA). Secondary antibodies were
obtained from LI-COR (IRDye 680 and 800 nm, for use with the LI-COR Odyssey system [Lincoln, NE])
and Santa Cruz Biotechnology (horseradish peroxidase conjugated).

Dersh D., Jones S.M., Eletto D., Christianson J.C, & Argon Y. (2014). OS-9 facilitates turnover of nonnative GRP94 marked by hyperglycosylation. Molecular Biology of the Cell, 25(15), 2220-2234.

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Western Blot Antibody Reagents

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Anti-firefly luciferase (Abcam, Cambridge, UK; ab187340, 1:4000), anti-GAPDH (CST, Danvers, USA; #2118, 1:1000), anti-calnexin (Stressgen, San Diego, USA; SPA-860, 1:1000), anti-Sec61α (Sigma Aldrich, St. Louis, USA; SAB2500917, 1:1000), anti-β-actin (CST, #4970, 1:1000), anti-FLAG M2 (Sigma Aldrich, St. Louis, USA; F1804, 1:1000), anti-α-1antitrypsin (DAKO, Glostrup, Denmark; A0012, 1:1000), anti-IRE1α (CST, #3294, 1:1000), anti-PERK (CST, #3192, 1:500), anti-CHOP (CST, #2895T, 1:500), anti-IgG (H+L chain) (Rabbit) pAb-horseradish peroxidase (HRP) (MBL, Tokyo, Japan; 458, 1:5000), peroxidase AffiniPure goat anti-mouse IgG (H+L) (Jackson Immuno Research Laboratories, West Grove, USA; 115-035-003, 1:5000), anti-rabbit IgG, HRP-linked antibody (CST, 7074, 1:3000), and anti-mouse IgG, HRP-linked antibody (CST, 7076, 1:3000) were purchased. Anti-Sec61β (1:1000) was a kind gift from Dr Tom A. Rapoport (Harvard Medical School, Boston, USA). Anti-ERP57 antibody (1:1000) was generated in our laboratory (Sopha et al., 2012) .

Sogawa A., Komori R., Yanagitani K., Ohfurudono M., Tsuru A., Kadoi K., Kimata Y., Yoshida H, & Kohno K. (2023). Signal sequence-triage is activated by translocon obstruction sensed by an ER stress sensor IRE1α. Cell structure and function, 48(2).

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