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Jcb117

Manufactured by Agilent Technologies

The JCB117 is a laboratory centrifuge designed for general-purpose applications. It features a compact and durable construction, and can accommodate a variety of sample types and rotor configurations.

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2 protocols using jcb117

1

Immunohistochemical Analysis of Tissue Samples

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Tissue specimens were fixed in 10% formaldehyde, embedded in paraffin, and cut into 3‐μm thick sections. Each section was further stained with hematoxylin and eosin. Immunohistochemical staining was performed on paraffin sections using antibodies against CD3 (1:200, LN10; Novocastra), CD5 (1:100, 4C7; Novocastra), CD10 (1:100, 56C6; Novocastra), CD15 (1:50, Carb‐3; DAKO), CD20 (1:100, L26; DAKO), CD30 (1:40, Ber‐H2; DAKO), CD79a (1:100, JCB117; DAKO), Ki‐67 (1:2500, MIB‐1; DAKO), and PD‐L1 (1:400, E1LN3; Cell Signaling Technology) and samples were analyzed using a Bond III Stainer (Leica Biosystems).
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2

Immunohistochemical Analysis of Lymphoma

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Biopsies were fixed in 10% neutral buffered formalin, embedded in paraffin, and stained with hematoxylin-eosin. Immunohistochemical staining and in situ hybridization were performed on an automated stainer (BOND-MAX; Leica Biosystems). The presence of EBV was demonstrated by in situ hybridization with a probe for small RNA–encoding regions 1 and 2 (EBERs) probe (Dako). Antibodies used were anti-CD3 (polyclonal rabbit, CD3ε, A0452; Dako, Agilent Technologies), CD4 (monoclonal IgG1k mouse, NCL-L-CD4-368; Leica Biosystems), CD8 (monoclonal mouse IgG1k, C8/144B; Dako), CD20 (monoclonal mouse IgG2ak, L26; Dako), and anti-CD79a (monoclonal mouse IgG1k, JCB117; Dako).
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