The breast cancer sample tissue microarrays (BC08118) for IHC analysis were purchased from Biomax (Rockville, MD, USA). The same tissue samples were stained with RBM38 and ZO-1 antibody respectively. The RBM38 antibody (LifeSpan Biosciences, Seattle, WA, USA) was used at the dilution of 1 : 300. The ZO-1 antibody (Abcam, Cambridge, MA, USA) was used with a concentration of 10 μg ml−1. The IHC staining was conducted and analysed as previously described (Shi et al, 2015 (link)).
Rbm38 antibody
Manufactured by LifeSpan BioSciences
Sourced in United States
The RBM38 antibody is a research tool used to detect and study the RNA-binding protein RBM38 (RNA-binding motif protein 38) in biological samples. RBM38 is involved in various cellular processes, including mRNA stability and cell cycle regulation. This antibody can be utilized in techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to investigate the expression and localization of RBM38 in different cell types and tissues.
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3 protocols using rbm38 antibody
1
Immunohistochemical Analysis of Breast Cancer
2
IHC Staining of Tissue Microarrays
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The IHC staining of the tissue microarrays was performed as previously described [43 (link)-44 (link)]. The same tissue samples were stained with RNPC1a and ERα antibody respectively. The RBM38 antibody (LifeSpan Biosciences, USA) was used at the dilution of 1:350. The ERα antibody (Cell Signaling technology, USA) was used at the dilution of 1:300. The rabbit polyclonal antibody was used as anti-RBM38 and ERα primary antibody.
3
Immunohistochemical Analysis of RBM38
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Serial formalin-fixed and paraffin-embedded tissues were sectioned at a 4-µm thickness, deparaffinized, and rehydrated in gradients of high-percentage ethanol to distilled water. For quenching endogenous peroxidase activity, sections were immersed in 3% hydrogen peroxide for 15 min at room temperature. Antigen retrieval involved boiling in 10 mM sodium citrate buffer (pH 6.0) for 3 min in a pressure cooker, followed by cooling to room temperature. Sections were then incubated with the RBM38 antibody (diluted 1:350; Life Span Biosciences, USA) at 4°C overnight, After washing in TBST, slides were incubated with the anti-RBM38 rabbit IgG antibody (Vector Laboratories, Burlingame, CA, USA) for 30 min at room temperature, followed by 3,3′-diaminobenzidine tetrahydrochloride (DAB) staining. Sections were lightly counterstained with hematoxylin.
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