Cast 2

Manufactured by Olympus

Sourced in Belgium

The CAST-2 is a versatile laboratory equipment designed for sample preparation and analysis. It provides precise control over temperature, pressure, and other critical parameters to facilitate various experimental procedures. The CAST-2 is a well-engineered instrument that helps researchers and scientists conduct their work effectively and efficiently.

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Lab products found in correlation

Integrator system software, by Visiopharm (1 mentions) Bx51 microscope, by Visiopharm (1 mentions) Bx50 microscope, by Olympus (1 mentions)

Close spelling variants of this product

CAST) 2.0 system CAST‐Grid 2.1.5 CAST stereology software version 2.3.1.5

2 protocols using cast 2

Immunohistochemical Analysis of ErbB4 Expression

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Animal perfusion and brain processing were performed as previously described64 (link). Immunohistochemistry was performed on 7 μm coronal sections as described in19 (link), 64 (link) using the anti-ErbB4 primary antibodies (1:400; Chemicon, HFR1/2G4), followed by Alexa Fluor-conjugated (Invitrogen) secondary antibodies. Negative controls were prepared identically, but the primary antibody was omitted.
Images were acquired using an Olympus BX-51 microscope and the Visiopharm Integrator System software (Visiopharm). Quantifications were performed in the sublesion area (3 sections per animal, Sham/KO/WT, n =4/6/8) using the computer-assisted Stereological Toolbox program with unbiased sampling (CAST-2, Olympus) by a researcher blind to treatment.

Pankratova S., Klingelhofer J., Dmytriyeva O., Owczarek S., Renziehausen A., Syed N., Porter A.E., Dexter D.T, & Kiryushko D. (2018). The S100A4 Protein Signals through the ErbB4 Receptor to Promote Neuronal Survival. Theranostics, 8(14), 3977-3990.

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Analyzing Ovarian Follicle Degeneration

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The grafts were analyzed using systematically randomly retained (i.e. every seventh section after slicing the tissue block at a random start position) sections that were processed for immunohistochemistry. For the analyses of the vWF and activated caspase-3 stained tissue sections, an Olympus BX50 microscope connected to a computer running the software program CAST 2 (Olympus Belgium) was used. One single investigator performed the analyses blinded to treatment (VEGF + , VEGF -or CTL) and transplantation period (2 or 4 weeks). If 70% of the surrounding granulosa cells were defined as 'activated caspase-3immunoreactive (IR)' (stained red), the entire follicle was considered 'dead'. The number of 'dead' follicles versus the total number of follicles was counted and their ratio was calculated (Figure 3). No valid recordings from the VEGF + treatment were obtained in OV5, therefore data from this ovary are excluded for all further analyses. Ovaria numbers 6 to 8 were renumbered into ovaria 5 to 7.

Langbeen A., Ginneken C.V., Fransen E., Bosmans E., Leroy J.L.M.R, & Bols P.E.J. (2016). Morphometrical analysis of preantral follicular survival of VEGF-treated bovine ovarian cortex tissue following xenotransplantation in an immune deficient mouse model. Animal reproduction science, 168.

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