PLA was performed with the Duolink Proximity Ligation Assay kit according to the manufacturer’s instructions (Sigma). Fixation was performed as in the normal immunostaining procedure. For the permeabilization step, 0.5% Triton X-100 in PBS was used in two washes of 20 min each. Antibodies used for PLA include rabbit anti-GFP (1:200; ChromoTek # PABG1-20, RRID:AB_2749857 ) and mouse α-Spec (1:50, DSHB, 3A9, RRID:AB_528473 ) antibodies, and secondary anti-mouse MINUS (Sigma-Aldrich Cat# DUO92004, RRID:AB_2713942 ) and anti-rabbit PLUS (Sigma-Aldrich Cat# DUO92002, RRID:AB_2810940 ) probes were used.
Pabg1 20
Manufactured by Proteintech
Sourced in United Kingdom
PABG1-20 is a 20-well Protein A Binding Plate designed for rapid parallel purification of antibodies and antibody fragments from small sample volumes. The plate is made of polypropylene and is compatible with standard 96-well microplate equipment.
Automatically generated - may contain errors
2 protocols using pabg1 20
1
Proximity Ligation Assay for Protein Interactions
2
Immunofluorescence Antibody Staining Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The following primary antibodies were used for immunofluorescence: anti-Ki-67 (1:100, mouse monoclonal, BD Transduction laboratory, Oxford, UK, 610968); anti-nucleolin (1:300, rabbit polyclonal; Abcam, ab22758); anti-α-tubulin antibody (1:10,000, Sigma-Aldrich, B512), anti-GFP (1:1000, Invitrogen, A-11122); anti-lamin A/C (1:2500, Abcam, ab108595) and anti-GFP (1:2000, ChromoTek, PABG1-20). The anti-centromere antibody (1:5000) comes from the collection of W.C.E.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!