Fam13a hpa038109

Manufactured by Merck Group

The FAM13A (HPA038109) is a laboratory reagent produced by Merck Group. It is a protein-coding gene that is used in various research applications. This product provides researchers with a tool to study the function and expression of the FAM13A gene.

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Lab products found in correlation

β actin mab1501, by Merck Group (2 mentions) C ebpα sc 61, by Santa Cruz Biotechnology (2 mentions) Imagequant tl, by GE Healthcare (2 mentions) Amersham imager 600, by GE Healthcare (2 mentions) Gapdh 60004 1 ig, by Proteintech (2 mentions) Plin1 gp29, by Progen Biotechnik (2 mentions) Bradford protein assay, by Bio-Rad (1 mentions) Bca protein assay, by Bio-Rad (1 mentions)

2 protocols using fam13a hpa038109

Western Blot Analysis of Cellular Proteins

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Tissues and cells were homogenized and lysed in lysis buffer as previously described [19 (link)]. The protein concentration was determined by Bradford protein assay (Bio-Rad). Equal amounts of proteins were loaded and immunoblot analysis was carried out according to standard protocol. The blots were visualized using the ECL chemiluminescence system by Amersham Imager 600 (GE healthcare) and quantified by densitometric analysis using ImageQuantTL (GE healthcare). The following antibodies were used: rabbit polyclonal antibodies against FAM13A (HPA038109; Sigma Life Science); PPARγ (2435), β-Catenin (8480), IRS1 (2382), GSK3β (12456), pGSK3β^S9 (9336), SIRT1 (9475) from Cell Signaling Technology; GAPDH (60004-1-IG, Proteintech); PLIN1 (GP29; Progen Biotechnik GmbH); ATGL (10006409; Cayman Chemical); C/EBPα (sc-61, Santa Cruz); β-ACTIN (MAB1501) from Millipore.

Tang J., Zhou H., Sahay K., Xu W., Yang J., Zhang W, & Chen W. (2018). Obesity-associated family with sequence similarity 13, member A (FAM13A) is dispensable for adipose development and insulin sensitivity. International Journal of Obesity (2005), 43(6), 1269-1280.

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Immunoblot analysis of adipogenic proteins

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Tissues and cells were homogenized and lysed in lysis buffer as previously described^{19 (link)}. The protein concentration was determined by BCA protein assay (Bio-rad). Equal amounts of proteins were loaded and immunoblot analysis was carried out according to standard protocol. The blots were visualized using the ECL chemiluminescence system by Amersham Imager 600 (GE healthcare) and quantified by densitometric analysis using ImageQuantTL (GE healthcare). The following antibodies were used: rabbit polyclonal antibodies against FAM13A (HPA038109; Sigma Life Science); PPARγ (2435), β-Catenin (8480), IRS1 (2382), GSK3β (12456), pGSK3β^S9 (9336), SIRT1 (9475) from Cell Signaling Technology; GAPDH (60004–1-IG, Proteintech); PLIN1 (GP29; Progen Biotechnik GmbH); ATGL (10006409; Caymen Chemicals); C/EBPα (sc-61, Santa Cruz); β-ACTIN (MAB1501) from Millipore.

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