Hra 1

Manufactured by Heidelberg Engineering

Sourced in Germany

The HRA 1 is a versatile lab equipment product from Heidelberg Engineering. It is designed to perform high-resolution analysis tasks. The core function of the HRA 1 is to provide accurate and reliable data for research and diagnostic applications.

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Lab products found in correlation

3d oct 2000 spectral domain, by Topcon (1 mentions) Retiport erg system, by Roland Consult (1 mentions) Red549 kit, by Hypoxyprobe (1 mentions) Micron 3 system, by Phoenix Pharmaceuticals (1 mentions) Ab6586, by Abcam (1 mentions)

4 protocols using hra 1

Clinical Evaluation and Genetic Analysis of STGD

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Probands and other family members were ascertained primarily at Peking Union Medical College Hospital (PUMCH, Beijing, China). Medical and family histories were recorded. Detailed ophthalmologic examinations, including visual acuities, color vision test (pseudoisochromatic plates and D-15 color plates), slit lamp biomicroscopy, tonometry and dilated ophthalmoscopy were conducted. Macular structure was examined with optical coherence tomography (OCT) (3D OCT-2000 Spectral Domain; Topcon, Tokyo, Japan). Auto fluorescence images (HRA 1; Heidelberg Engineering, Heidelberg, Germany) were obtained. Full-field ERGs were performed (RetiPort ERG system, Roland Consult, Wiesbaden, Germany) in selected patients. The method was performed in concordance with the International Society for Clinical Electrophysiology of Vision standard protocol (ISCEV).
Diagnosis of STGD was based on the clinical manifestations. Written informed consents were obtained from participants or their guardians. Genomic DNA was isolated from peripheral leukocytes using a commercial kit (QIAamp Blood Midi; Qiagen, Hilden, Germany) according to manufacturer's protocol. This study was approved by the Institutional Review Board of PUMCH and adhered to the tenets of the Declaration of Helsinki and the Guidance on Sample Collection of Human Genetic Diseases by the Ministry of Public Health of China.

Zaneveld J., Siddiqui S., Li H., Wang X., Wang H., Wang K., Li H., Ren H., Lopez I., Dorfman A., Khan A., Wang F., Salvo J., Gelowani V., Li Y., Sui R., Koenekoop R, & Chen R. (2014). Comprehensive analysis of Stargardt macular dystrophy patients reveals new genotype-phenotype correlations and unexpected diagnostic revisions. Genetics in medicine : official journal of the American College of Medical Genetics, 17(4), 262-270.

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Retinal Imaging with SLO Angiography

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Retinal structures of the anesthetized animals were visualized via SLO imaging with a HRA 1 and HRA 2 (Heidelberg Engineering, Heidelberg, Germany) according to previously described procedures [18] . Briefly, HRA 1 and HRA 2 systems feature lasers in the short (visible) wavelength range (488 nm in both and 514 nm in HRA 1 only), and also in the long (infrared) wavelength range (795/830 nm and 785/815 nm). The 488 and 795 nm lasers are used for fluorescein (FLA) and indocyanine green (ICG) angiography, respectively.

Garcia Garrido M., Beck S.C., Mühlfriedel R., Julien S., Schraermeyer U, & Seeliger M.W. (2014). Towards a Quantitative OCT Image Analysis. PLoS ONE, 9(6), e100080.

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Multimodal Imaging of Retinal Hypoxia

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Fundus images were taken with a Micron III system (Phoenix Research Labs, Pleasanton, CA, USA). Angiography was performed with rhodamine B (100 mg/ml, 200 mg/kg mouse, i.p.) that nicely fits to the TRITC filter of the Micron system, or with fluorescein and a SLO (HRA1, Heidelberg Engineering, Heidelberg, Germany).
Hypoxic retinal areas were visualized by the Hypoxyprobe Red549 Kit (Hypoxyprobe, Burlington, MA, USA). In brief, pimonidazol was dissolved at a concentration of 20 mg/ml and used intraperitoneally at 60 mg/kg. After 3 h, mice were perfused with 2% paraformaldehyde. Retinal flatmounts were prepared and stained with an antibody raised against hypoxyprobe followed by an antibody raised against Col4 (1:250, polyclonal, ab6586, Abcam, Cambridge, UK) or by staining with lectin (10 μg/ml FITC-lectin (BSI) from Griffonia simplicifolia, L9381, Sigma, Taufkirchen, Germany) in order to stain vessels.
Paraffin sections were prepared for histological examination by standard methods after formalin fixation, paraffin embedding and staining with hematoxylin and eosin (HE).

Martin G., Conrad D., Cakir B., Schlunck G, & Agostini H.T. (2018). Gene expression profiling in a mouse model of retinal vein occlusion induced by laser treatment reveals a predominant inflammatory and tissue damage response. PLoS ONE, 13(3), e0191338.

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Retinal Imaging of Anesthetized Mice

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The retinal structures of the anesthetized mice were visualized via cSLO imaging with HRA1 and HRA2 systems (Heidelberg Engineering) according to previously described procedures^{22 (link)}. Briefly, HRA1 and HRA2 systems feature lasers in the short (visible) wavelength range (488 nm for both and 514 nm for HRA1 only) and also in the long (infrared) wavelength range (795/830 nm and 785/815 nm, respectively). The 488- and 795-nm lasers are used for fluorescein (FLA) and indocyanine green (ICG) angiography, respectively.

Kohl S., Zobor D., Chiang W.C., Weisschuh N., Staller J., Menendez I.G., Chang S., Beck S.C., Garrido M.G., Sothilingam V., Seeliger M.W., Stanzial F., Benedicenti F., Inzana F., Héon E., Vincent A., Beis J., Strom T.M., Rudolph G., Roosing S., den Hollander A.I., Cremers F.P., Lopez I., Ren H., Moore A.T., Webster A.R., Michaelides M., Koenekoop R.K., Zrenner E., Kaufman R.J., Tsang S.H., Wissinger B, & Lin J.H. (2015). Mutations in the unfolded protein response regulator ATF6 cause the cone dysfunction disorder achromatopsia. Nature genetics, 47(7), 757-765.

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