Cd11b

Manufactured by FlowJo

Sourced in United States

CD11b is a cell surface glycoprotein that functions as an integrin. It is expressed on the surface of various immune cells, including monocytes, macrophages, granulocytes, and natural killer cells. CD11b plays a crucial role in cell adhesion, migration, and phagocytosis. It is commonly used as a marker for the identification and characterization of these immune cell populations.

Automatically generated - may contain errors

Lab products found in correlation

Cd11b, by BioLegend (2 mentions) Facsaria 3, by BD (1 mentions) Cd11b bv421, by BioLegend (1 mentions) Adult brain dissociation kit, by Miltenyi Biotec (1 mentions) Countbright absolute counting beads, by Thermo Fisher Scientific (1 mentions) Propidium iodide, by Miltenyi Biotec (1 mentions) Perm wash buffer, by BD (1 mentions) Cd11b, by Thermo Fisher Scientific (1 mentions) Cytofix cytoperm fixation permeabilization kit, by BD (1 mentions) Facscaliber four color cytometer, by BD (1 mentions) Cd106, by BioLegend (1 mentions)

4 protocols using cd11b

Quantifying Microglial Phagocytosis of Myelin and Amyloid-β

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were taken down 2 days after antibody dosing, and brains were dissected after PBS perfusion and dissociated with the Adult Brain Dissociation Kit (Miltenyi Biotec, 130-107-677), according to the manufacturer’s protocol. Microglia number was measured by FACS using CountBright Absolute Counting Beads (Invitrogen, C36950) and diluted to 500 microglia per µl in DPBS + 0.5% BSA. The resulting cell suspension was mixed with pHrodo-green labeled myelin (50 µg ml⁻¹ in DPBS + 0.5% BSA) or FAM-Aβ (200 nM in DPBS + 0.5% BSA) and incubated at 37 °C for 45 minutes with gentle mixing. Cell suspensions were washed and stained with the following antibodies in FACS buffer (1% fatty acid-free BSA and 1 mM EDTA in PBS) for 25 minutes on ice: CD11b-BV421 (BioLegend, 101251) and mouse Fc blocker (anti-mouse CD16/32, BioLegend, 101320). Cells were washed with FACS buffer, resuspended in FACS buffer with propidium iodide (Miltenyi Biotec, 130-93-233), strained through a 100-μm filter and then analyzed on a flow cytometer (BD FACSAria III). FCS files were then imported and analyzed in FlowJo software (version 10).The percentage of myelin⁺ microglia (pHrodo-green⁺, CD11b⁺) and Aβ⁺ microglia (FAM⁺, CD11b⁺) in the total CD11b⁺ microglial population was calculated. See Supplementary Methods for details on myelin debris and Aβ fibril preparation and fluorescent labeling.

van Lengerich B., Zhan L., Xia D., Chan D., Joy D., Park J.I., Tatarakis D., Calvert M., Hummel S., Lianoglou S., Pizzo M.E., Prorok R., Thomsen E., Bartos L.M., Beumers P., Capell A., Davis S.S., de Weerd L., Dugas J.C., Duque J., Earr T., Gadkar K., Giese T., Gill A., Gnörich J., Ha C., Kannuswamy M., Kim D.J., Kunte S.T., Kunze L.H., Lac D., Lechtenberg K., Leung A.W., Liang C.C., Lopez I., McQuade P., Modi A., Torres V.O., Nguyen H.N., Pesämaa I., Propson N., Reich M., Robles-Colmenares Y., Schlepckow K., Slemann L., Solanoy H., Suh J.H., Thorne R.G., Vieira C., Wind-Mark K., Xiong K., Zuchero Y.J., Diaz D., Dennis M.S., Huang F., Scearce-Levie K., Watts R.J., Haass C., Lewcock J.W., Di Paolo G., Brendel M., Sanchez P.E, & Monroe K.M. (2023). A TREM2-activating antibody with a blood–brain barrier transport vehicle enhances microglial metabolism in Alzheimer’s disease models. Nature Neuroscience, 26(3), 416-429.

+ Open protocol

+ Expand

Flow Cytometric Analysis of Splenic CD11b and GC Receptor

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single cell suspensions of splenocytes were used for flow cytometric analyses of CD11b (eBioscience, San Diego, CA) and GC receptor (Santa Cruz Biotechnology, Inc., Dallas, TX) expression (n = 6/group). In brief, washed splenocytes, blocked FcγII/III receptors (BD Biosciences, San Jose, CA) for 15 min at 4 °C, and washed the cells. Cells were incubated with CD11b antigen for 30 min at 4 °C and washed 2 times. Resuspended cells were fixed and permeabilized by BD Cytofix/Cytoperm™ Fixation/Permeabilization kit (BD Biosciences, San Jose, CA), followed by 2-time wash in 1X BD Perm/Wash™ buffer. Cells resuspended were incubated with GC receptor antigen for 30 min at 4 °C and washed 2 times with 1X BD Perm/Wash™ buffer. Because the GC receptor primary antibody is not conjugated, splenocytes were incubated in secondary antibody (PE; goat anti-mouse IgG1-PE, Santa Cruz Biotechnology, Inc., Dallas, TX). Non-specific, isotype-matched antibodies were used to assess nonspecific binding in combination with unstained cells and blocking control. A Becton-Dickinson FACSCaliber four color cytometer was used to determine antigen expression, and 10,000 events were recorded for each sample and control. FlowJo software (ver. 7.6, Ashland, OR) was used to analyze data, and gating for CD11b and GC receptor was determined based on non-specific binding of appropriate negative isotype stained controls.

Jung S.H., Wang Y., Kim T., Tarr A., Reader B., Powell N, & Sheridan J.F. (2014). Molecular Mechanisms of Repeated Social Defeat-Induced Glucocorticoid Resistance: Role of microRNA. Brain, behavior, and immunity, 44, 195-206.

+ Open protocol

+ Expand

Monocyte and Macrophage Profiling in Polycystic Kidney Disease

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tibia bones and spleens were isolated from PKD1-PKD3^fl/fl and PKD1-PKD3^Δmy mice. Bone marrow cells were flushed out and spleens were homogenized with 1× PBS containing 1% BSA. 2 × 10⁵ cells were used and blocked with antibody against CD32/16 (553141; BD Biosciences) for 5 min at room temperature, followed by staining with antibodies against CD11b (101225; BioLegend), CD11c (561045; BD PharMingen), CD115 (135505; BioLegend), Ly6c (128015; BioLegend), CD117 (105813; BioLegend), CX3CR1 (149009; BioLegend) for 20 min on ice in the dark. After washing, cells were analyzed by FACS (LSRII; BD). Dead cells were excluded by DAPI staining. Monocytes and macrophages were identified as CD11b⁺CD11c⁻Ly6c⁺CD115⁺ using FlowJo. Bone marrow derived monocyte/DC progenitors were identified as CD11b⁻CD11c⁻CD115⁺CD117⁺Cx3CR1⁺. Counts of monocytes and macrophages were shown as percentage of counted intact cells.

Zhang Z., Meszaros G., He W.T., Xu Y., de Fatima Magliarelli H., Mailly L., Mihlan M., Liu Y., Puig Gámez M., Goginashvili A., Pasquier A., Bielska O., Neven B., Quartier P., Aebersold R., Baumert T.F., Georgel P., Han J, & Ricci R. (2017). Protein kinase D at the Golgi controls NLRP3 inflammasome activation. The Journal of Experimental Medicine, 214(9), 2671-2693.

+ Open protocol

+ Expand

Characterization of Mesenchymal Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

When the stem cells were passaged to the fifth generation, the cell phenotype was identified by flow cytometry. After trypsinization, the cells were centrifuged at 1000 rpm for 10 min and washed twice with PBS. After resuspension in PBS, the cells were centrifuged at 2000 rpm for 6 min, and the supernatant was discarded. One microliter of antibody was added to each tube, and was incubated for 30 min at 4 °C in the dark. Then, 1 mL PBS was added to each tube and centrifuged at 2000 rpm for 6 min. This step was repeated twice. Finally, the cells were resuspended in 0.5 mL PBS and analyzed using flow cytometry. The antibodies included positive markers (CD29 (catalog #102207, Biolegend, San Diego, CA, USA), CD90 (catalog #202503, Biolegend, San Diego, CA, USA), CD106 (catalog #200403, Biolegend, USA), and negative markers, CD11b (catalog #201807, Biolegend, San Diego, CA, USA), and CD45 (catalog #202207, Biolegend, San Diego, CA, USA). According to FlowJo software (7.6.5, Ashland, Wilmington, DE, USA) analysis, CD29+, CD90+, and CD106+ cells were designated as MSCs, and CD45+ and CD11b+ cells were designated as hematopoietic stem cells.

Wang X., He R., Nian S., Xiao B., Wang Y., Zhang L., Wang X., Guo R, & Lu Y. (2022). Treatment of Pelvic Organ Prolapse by the Downregulation of the Expression of Mitofusin 2 in Uterosacral Ligament Tissue via Mesenchymal Stem Cells. Genes, 13(5), 829.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!