PBMC were isolated from whole EDTA blood by Ficoll-Paque Plus (GE Healthcare) gradient centrifugation. Mononuclear cell preparations of intestinal biopsy and necropsy tissues were obtained by mechanical disruption and enzymatic dissociation followed by lymphocyte separation by centrifugation through Ficoll-Paque Plus (Amersham BioScience), as described previously54 (link). Freshly isolated cells were immunophenotyped using the following antibody panel: CD4 Pacific Blue (clone OKT4; BioLegend; 1:50), CCR5 PE (clone 3A9; BD Biosciences; 1:20), CD28 ECD (clone CD28.2; Beckman Coulter, 1:20), CD95 PE-Cy5 (clone DX2; BD Biosciences; 1:10), CD8 PE-Cy7 (clone SK1: BD Biosciences; 1:100), CD38 APC (clone OK10; NIH Nonhuman Primate Reagent Resource; 1:25), CD3 APC-Cy7 (clone SP34-2; BD Biosciences; 1:100) and Ki67 FITC (clone B56; BD Biosciences; 1:20). Approximately 100,000 CD3+ T cells were acquired for each sample and data analysis was performed using FCS Express software. All dilutions are from the manufacturer’s stock and based on 100 μl total staining volume.
Cd8 pe cy7 clone sk1
Manufactured by BD
The CD8 PE-Cy7 (clone SK1) is a fluorochrome-conjugated monoclonal antibody that binds to the CD8 surface antigen on human T lymphocytes. It is used in flow cytometry applications to identify and enumerate CD8-positive cells.
Automatically generated - may contain errors
Lab products found in correlation
Ccr5 pe clone 3a9, by BD (2 mentions)
Cd95 pe cy5 clone dx2, by BD (2 mentions)
Cd3 apc cy7 clone sp34 2, by BD (2 mentions)
Ki 67 fitc clone b56, by BD (2 mentions)
Cd4 pacific blue clone okt4, by BioLegend (2 mentions)
Cd28 ecd clone cd28 2, by Beckman Coulter (2 mentions)
Ficoll paque plus, by GE Healthcare (2 mentions)
Ficoll paque plus, by Cytiva (1 mentions)
Cd3 apc h7 clone sk7, by BD (1 mentions)
Cd4 pe clone sk3, by BD (1 mentions)
3 protocols using cd8 pe cy7 clone sk1
1
Comprehensive PBMC Isolation and Immunophenotyping
2
Immunophenotyping of Peripheral Blood and Tissue Lymphocytes
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Peripheral blood mononuclear cells were isolated from whole EDTA blood by Ficoll-Paque Plus (GE Healthcare) gradient centrifugation. Mononuclear cell preparations of intestinal biopsy and necropsy tissues were obtained by mechanical disruption and enzymatic dissociation followed by lymphocyte separation by centrifugation through Ficoll-Paque Plus (GE Healthcare), as described previously54 (link). Freshly isolated cells were immunophenotyped using the following antibody panel: CD4 Pacific Blue (clone OKT4; BioLegend; 1:50); CCR5 PE (clone 3A9; BD Biosciences; 1:20); CD28 ECD (clone CD28.2; Beckman Coulter, 1:20); CD95 PE-Cy5 (clone DX2; BD Biosciences; 1:10); CD8 PE-Cy7 (clone SK1: BD Biosciences; 1:100); CD38 APC (clone OK10; NIH Nonhuman Primate Reagent Resource; 1:25); CD3 APC-Cy7 (clone SP34-2; BD Biosciences; 1:100); and Ki67 FITC (clone B56; BD Biosciences; 1:20). Approximately 100,000 CD3+ T cells were acquired for each sample and data analysis was performed using FCS Express software. All dilutions are from the manufacturer's stock and based on 100 μl total staining volume.
3
Mouse Anti-Human Flow Cytometry Antibodies
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