Cyclin d1 ab6152 antibody

RPMI-1640 medium, 0.05% trypsin-EDTA, and penicillin-streptomycin solution were obtained from Gibco (Thermo Fisher Scientific, Inc., Waltham, MA, USA). Silibinin and fetal bovine serum (FBS) were purchased from Sigma-Aldrich (Merck KGaA, St. Louis, MO, USA). Antibodies specific for VEGF (sc-507), STAT5b (sc-1656), CDK4 (sc-260), MMP9 (sc-13520), cyclin E (sc-481), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; #2118) together with their specific secondary antibodies (anti-mouse (sc-516102) and anti-rabbit (sc-2357)) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Antibodies specific for p27 (#3686), p21 (#2974), phosphorylated epidermal growth factor (EGF) receptor (pEGFR; #3777), EGFR (#4267), phosphorylated JAK2 (pJAK2; (#3776) and JAK2 (#3230)), and phosphorylated STAT5 (pSTAT5; #9351) were purchased from Cell Signaling Technology (Beverly, MA, USA). Cyclin D1 (ab6152) antibody was obtained from Abcam (Cambridge, MA, USA). SOX2 (#MAB4423), NANOG (#MABD24), OCT4 (#MABD76), and MMP3 (#AB2963) were purchased from Merck Millipore (Burlington, MA, USA). The MMP2 (E90317) antibody was obtained from EnoGene (New York, NY, USA). The antibody specific for PD-L1 (R30949) was purchased from NSJ Bioreagents (San Diego, CA, USA).

Roswell Park Memorial Institute-1640 (RPMI-1640) medium, penicillin–streptomycin solution, and trypsin-EDTA (0.05%) (Gibco, Thermo Fisher Scientific, Inc., Waltham, MA, USA) were purchased. UA (U6753) and fetal bovine serum (FBS) (Sigma-Aldrich, Merck KGaA, St. Louis, MO, USA) were obtained. The primary antibodies against CDK4 (sc-260), cyclin E (sc-481), VEGF (sc-507), MMP9 (sc-13520), and β-actin (sc-47778) with anti-mouse (sc-516102) and anti-rabbit (sc-2357) secondary antibodies (Santa Cruz Biotechnology, Dallas, TX, USA) were procured. The antibodies against p21 (#2974), p27 (#3686), pEGFR (#3777), EGFR (#4267), pJAK2 (#3776), JAK2 (#3230), pSTAT3 (#9145), and STAT3 (#9139) (Cell Signaling Technology, Beverly, MA, USA) were obtained. The antibodies against SOX2 (#MAB4423), OCT4 (#MABD76), NANOG (#MABD24), and MMP3 (#AB2963) were supplied by Merck Millipore (Burlington, MA, USA). The Cyclin D1 (ab6152) antibody (Abcam, Cambridge, MA, USA), MMP2 (E90317) antibody (EnoGene, New York, NY, USA), and the PD-L1 (R30949) antibody (NSJ Bioreagents, San Diego, CA, USA) were procured.