Cathepsin B activity was measured according to the Enzymatic Assay of Cathepsin B protocol provided by sigma Inc. Briefly, cell lysates were incubated with cathepsin B substrate Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin at 37°C, and the fluorescence intensity was determined at 440 nm in a fluorescence microplate reader under excitation at 348 nm.
Enzymatic assay of cathepsin b
Manufactured by Merck Group
The Enzymatic Assay of Cathepsin B is a laboratory instrument designed for the quantitative measurement of cathepsin B activity. Cathepsin B is a lysosomal cysteine protease enzyme that plays a role in various physiological and pathological processes. The assay provides a standardized method to determine the enzymatic activity of cathepsin B in biological samples.
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Lab products found in correlation
3 protocols using enzymatic assay of cathepsin b
1
Cathepsin B Activity Fluorometric Assay
2
Cathepsin B Activity Assay
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Cells or tissue were lysed. Cathepsin B activity was measured according to the Enzymatic Assay of Cathepsin B protocol provided by Sigma, Inc. Briefly, cell lysates were incubated with cathepsin B substrate Nα-CBZ-Arg-Arg-7-amido-4-methylcoumarin at 37°C, and the fluorescence intensity was determined at 440 nm in a fluorescence microplate reader under excitation at 348 nm. Purified cathepsin B protein was added to the reaction mixture separately as the standard.
3
Cathepsin B Enzymatic Activity Assay
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Cathepsin B activity was measured according to the Enzymatic Assay of Cathepsin B protocol provided by Sigma Inc. In brief, cell or tissue lysate was incubated with cathepsin B substrate Nα-CBZ-Arg-Arg-7-amido-4-methylcoumarin at 37 °C, and the fluorescence intensity was determined at 440 nm in a fluorescence microplate reader under excitation at 348 nm (Xiao et al. 2020 (link)).
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