Tumour samples were digested using Tumour Dissociation Kit human (Miltenyi Biotec). Labelled cells were analysed by BD LSRFortessa X‐20 cell analyser (BD Biosciences) and FlowJo v10 software (Tree Star). Anti‐CD20 antibodies and IgG2bκ isotype control were purchased from BD Biosciences or BioLegend. Anti‐CD19 antibodies, IgG2aκ isotype control and IgG1κ isotype control were obtained from BD Biosciences. Anti‐CD79b antibodies were purchased from Thermo Fisher Scientific or Miltenyi Biotec. Anti‐CD243 (anti‐MDR1) antibodies were obtained from BioLegend. IgG1 isotype control antibodies were purchased from Miltenyi Biotec. Mean fluorescence intensity (MFI) ratio was calculated by dividing the MFI value of stained cells by the MFI value of the respective isotype control‐stained cells.
Anti cd20 antibodies
Manufactured by BD
Sourced in United States
Anti-CD20 antibodies are laboratory reagents used in research applications. They specifically target the CD20 antigen, which is expressed on the surface of B cells. These antibodies can be used to identify and study B cell populations in various experimental settings.
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Lab products found in correlation
2 protocols using anti cd20 antibodies
1
Multiparametric Flow Cytometry of Tumour Samples
2
Establishing Resistant Cell Clones
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RL cells were pretreated with 300 µg/mL of mutagen N-ethyl-N-nitrosourea (ENU; Sigma-Aldrich) to establish resistant clones more efficiently by inducing mutagenesis [18 (link)], and were then treated with effector NK92 cells at an effector/target (E/T) ratio 20:1 and 0.1 μg/mL of obinutuzumab for 24 h. CD20 positive cells were enriched by magnetic-activated cell sorting using anti-CD20 antibodies (BD Biosciences, San Jose, CA, USA). These ADCC induction and cell sorting procedures were repeated three times, and regrown cells were then single cell cloned.
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