Rabbit anti cd81

Doxorubicin (DOX), indocyanine green (ICG), bovine serum albumin (BSA), tetraethylorthosilicate (TEOS), and cetyltrimethylammonium bromide (CTAB) were obtained from Sigma-Aldrich (United States). 3,3′-Dioctadecyloxacarbocyanine perchlorate (Dio) and 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) were bought from Biyuntian (China). Polyvinylidene fluoride membranes (PVDF) were acquired from Millipore (United States). MTT and Ki67 reagents were provided by Yeasen Corporation (China). RPMI 1640 medium, FBS, penicillin, and streptomycin were provided by Hyclone (United States). You Ning Wei Corporation (China) offered the other reagents, including acrylamide, mouse anti-MMP2 (Santa Cruz, United States), mouse anti-MMP9 (Santa Cruz, United States), rabbit anti-calnexin (Santa Cruz, United States), rabbit anti-CD81 (ProteinTech, Chicago, IL, United States), and rabbit anti-CD63 (Abcam, Cambridge, Britain).

The human CM line AC16 was obtained from the cell bank of the Chinese Academy of Science (Shanghai, China). The bicinchoninic acid assay was used to determine the total protein quantity from AC16 cells and exosomes. The proteins were separated on sodium dodecyl sulphate gel and transferred onto the polyvinylidene fluoride membranes. The proteins on the membranes were blocked and probed with primary antibodies against rabbit anti‐CD9 (Proteintech), rabbit anti‐CD81 (Proteintech) and rabbit anti‐Calnexin (Proteintech) overnight at 4°C, followed by incubation with the secondary antibodies against HRP‐lablled sheep anti‐rabbit immunoglobulin (Ig) G (Proteintech) for 1 h at room temperature. Enhanced chemiluminescence (Proteintech) was used to visualize the bands.